Mouse hydroxyproline, Hyp ELISA Kit | Hyp elisa kit
Mouse hydroxyproline, Hyp ELISA Kit
Reactivity
Mouse
Synonyms
hydroxyproline, Hyp; N/A; Mouse hydroxyproline, Hyp ELISA Kit; Mouse hydroxyproline; Hyp ELISA Kit; hydroxyproline; Hyp; Hyp elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse Hyp. No significant cross-reactivity or interference between mouse Hyp and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
7.8 ng/ml-500 ng/ml
Sensitivity
< 1.95 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for Hyp elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Hyp has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hyp present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Hyp is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hyp bound in the initial step. The color development is stopped and the intensity of the color is measured.
