Mouse Insulin Like Growth Factor Binding Protein 7 (IGFBP7) ELISA Kit | IGFBP7 elisa kit

Mouse Insulin Like Growth Factor Binding Protein 7 (IGFBP7) ELISA Kit

Cat. Num. AAA13947

• Gene Names: Igfbp7; AGM; Fstl2; Mac25
• Reactivity: Mouse
• Synonyms: Insulin Like Growth Factor Binding Protein 7 (IGFBP7); N/A; Mouse Insulin Like Growth Factor Binding Protein 7 (IGFBP7) ELISA Kit; FSTL2; IGFBP-7v; MAC25; PSF; TAF; IGFBP-rP1; MAC25 protein; Prostacyclin-stimulating factor; PGI2-stimulating factor; Tumor-derived adhesion factor; IGFBP7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IGFBP7. No significant cross-reactivity or interference between IGFBP7 and analogues was observed.
• Sequence Length: 282
• Assay Type: Quantitative Sandwich
• Samples: Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Detection Range: 6.25-400pg/mL
• Sensitivity: < 2.34pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGFBP7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGFBP7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for IGFBP7 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGFBP7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IGFBP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IGFBP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IGFBP7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IGFBP7 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 226958445
• NCBI GeneID: 29817
• NCBI Accession #: NP_032074.3
• NCBI GenBank Nucleotide #: NM_008048.3
• UniProt Accession #: Q61581; O88812; Q9EQW0
• Molecular Weight: 28,969 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 7 isoform 2
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 7
• NCBI Official Symbol: Igfbp7
• NCBI Official Synonym Symbols: AGM; Fstl2; Mac25
• NCBI Protein Information: insulin-like growth factor-binding protein 7
• UniProt Protein Name: Insulin-like growth factor-binding protein 7
• UniProt Gene Name: Igfbp7
• UniProt Synonym Gene Names: Mac25; IBP-7; IGF-binding protein 7; IGFBP-7