Standard Curve (Sample)
(Mouse KLK1 PicoKine ELISA Kit standard curve)
Mouse KLK1 ELISA Kit | Klk1 elisa kit
Mouse KLK1 PicoKine ELISA Kit
Gene Names
Klk1; Kal; Klk6; KAL-B; mGk-6; Klk1b6; 0610007D04Rik
Reactivity
Mouse
Synonyms
KLK1; N/A; Mouse KLK1 PicoKine ELISA Kit; Kallikrein 1; Kallikrein1; Kallikrein-1; klk 1; Klk 6; Klk-1; Klk1; P06870; Pancreatic kallikrein; Klk1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse KLK1
Sequence Length
213
Samples
Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<12pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
(Mouse KLK1 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse KLK1 PicoKine ELISA Kit standard curve)
Related Product Information for Klk1 elisa kit
Principle of the Assay: The Mouse KLK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for KLK1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: I25-D261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin- Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse KLK1 amount of sample captured in plate.
Background: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Background: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,775 Da
NCBI Official Full Name
kallikrein-1 isoform 2
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
Klk1
NCBI Official Synonym Symbols
Kal; Klk6; KAL-B; mGk-6; Klk1b6; 0610007D04Rik
NCBI Protein Information
kallikrein-1
UniProt Protein Name
Kallikrein-1
UniProt Gene Name
Klk1
UniProt Synonym Gene Names
Klk-6; Klk6; mGK-6
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Product Notes
The Mouse Klk1 klk1 (Catalog #AAA46807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46807 ELISA Kit recognizes Mouse Klk1. It is sometimes possible for the material contained within the vial of "KLK1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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