Standard Curve (Sample)
(Mouse Kremen-1 PicoKine ELISA Kit standard curve)
Mouse KREMEN1 ELISA Kit | KREMEN1 elisa kit
Mouse Kremen-1 PicoKine ELISA Kit
Gene Names
Kremen1; Krm1; Kremen; AV002070
Reactivity
Mouse
Synonyms
KREMEN1; N/A; Mouse Kremen-1 PicoKine ELISA Kit; KREMEN; Kremen1; Kremen 1; Kremen-1; KRM1; Kremen protein 1; Q99N43; kringle containing transmembrane protein 1; KREMEN1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Kremen-1
Sequence Length
473
Samples
Cell culture supernates, serum and plasma(heparin, EDTA).
Assay Type
Sandwich
Detection Range
125pg/ml-8000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Standard Curve (Sample)
(Mouse Kremen-1 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse Kremen-1 PicoKine ELISA Kit standard curve)
Related Product Information for KREMEN1 elisa kit
Principle of the Assay: Mouse Kremen-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kremen-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: A20-G395) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kremen-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Kremen-1 amount of sample captured in plate.
Background/Introduction: Kremen protein 1 is a protein that in humans is encoded by the KREMEN1 gene. This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
Background/Introduction: Kremen protein 1 is a protein that in humans is encoded by the KREMEN1 gene. This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,673 Da
NCBI Official Full Name
kremen protein 1
NCBI Official Synonym Full Names
kringle containing transmembrane protein 1
NCBI Official Symbol
Kremen1
NCBI Official Synonym Symbols
Krm1; Kremen; AV002070
NCBI Protein Information
kremen protein 1
UniProt Protein Name
Kremen protein 1
UniProt Gene Name
Kremen1
UniProt Synonym Gene Names
Kremen
UniProt Entry Name
KREM1_MOUSE
Similar Products
Product Notes
The Mouse KREMEN1 kremen1 (Catalog #AAA46703) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46703 ELISA Kit recognizes Mouse KREMEN1. It is sometimes possible for the material contained within the vial of "KREMEN1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.