Standard Curve (Sample)
Mouse Laminin ELISA Kit | LAMC2 elisa kit
Laminin Mouse ELISA Kit
Gene Names
LAMC2; B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
Reactivity
Mouse
Applications
ELISA
Synonyms
Laminin; N/A; Laminin Mouse ELISA Kit; Mouse Laminin; LAMC2 elisa kit
Reactivity
Mouse
Assay Type
Sandwich
Samples
Mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants
Detection Range
156 pg/ml-10,000 pg/ml
Sensitivity
< 10 pg/ml
Applicable Applications for LAMC2 elisa kit
ELISA
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LAMC2 elisa kit
Background: Laminins are major proteins in the basal lamina, a protein network foundation for most cells and organs. Fifteen laminin trimers have been identified. The laminins are combinations of different alpha-, beta-, and gamma-chains. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. Recently, several publications have demonstrated that laminins can be used to culture cells, such as pluripotent stem cells, that are difficult to culture on other substrates. Rodin et al. showed that recombinant laminin 511 can be used to create a totally xeno-free and defined cell culture environment to culture human pluripotent ES cells and human iPS cells.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Laminin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Laminin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Laminin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Laminin can be calculated.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Laminin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Laminin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Laminin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Laminin can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
130,976 Da
NCBI Official Full Name
Laminin
NCBI Official Synonym Full Names
laminin, gamma 2
NCBI Official Symbol
LAMC2
NCBI Official Synonym Symbols
B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
NCBI Protein Information
laminin subunit gamma-2; BM600-100kDa; laminin B2t chain; CSF 140 kDa subunit; nicein subunit gamma; kalinin subunit gamma; ladsin 140 kDa subunit; epiligrin subunit gamma; cell-scattering factor 140 kDa subunit; large adhesive scatter factor 140 kDa subunit
UniProt Protein Name
Laminin subunit gamma-2
UniProt Gene Name
LAMC2
UniProt Synonym Gene Names
LAMB2T; LAMNB2; CSF 140 kDa subunit
UniProt Entry Name
LAMC2_HUMAN
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Product Notes
The Mouse LAMC2 lamc2 (Catalog #AAA58861) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA58861 ELISA Kit recognizes Mouse LAMC2. AAA Biotech's Laminin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the LAMC2 lamc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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