Standard Curve (Sample)
Mouse Leptin ELISA Kit | Leptin elisa kit
Mouse Leptin ELISA Kit
Reactivity
Mouse
Synonyms
Leptin; Mouse Leptin ELISA Kit; Leptin/LEP/LEPD/OBS/Obesity Homolog; Leptin elisa kit
Reactivity
Mouse
Specificity
Specifically recognize Leptin, no obvious cross reaction with other analogues
Sequence Length
167
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Leptin elisa kit
Background: Leptin is a hormone secreted by adipose tissue. Its main function is to regulate body weight and energy balance by affecting appetite and metabolic rate. Leptin acts through receptors in areas such as the hypothalamus and abdomen.The level of leptin usually increases with an increase in body fat, and is also influenced by many other factors, such as diet, exercise, physiology, and disease. Research has shown that both high and low levels of leptin may be associated with weight and energy balance-related issues, such as obesity and metabolic syndrome. Leptin is also related to many other physiological processes, such as immune function, reproductive function, and bone formation. Understanding the role and regulatory mechanisms of leptin is important for understanding the mechanisms of weight regulation, metabolic diseases, and other related diseases.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti Leptin antibody was precoated onto the 96-well plate. The biotin conjugated anti Leptin antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with Leptin conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of Leptin in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti Leptin antibody was precoated onto the 96-well plate. The biotin conjugated anti Leptin antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with Leptin conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of Leptin in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
leptin
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Product Notes
The Mouse Leptin (Catalog #AAA17413) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17413 ELISA Kit recognizes Mouse Leptin. It is sometimes possible for the material contained within the vial of "Leptin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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