Standard Curve (Sample)
Mouse MEP1B ELISA Kit | MEP1B elisa kit
Mouse MEP1B ELISA Kit
Gene Names
Mep1b; Mep-1b
Reactivity
Mouse
Synonyms
MEP1B; N/A; Mouse MEP1B ELISA Kit; Meprin A subunit beta; Endopeptidase-2; Meprin B; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; PABA peptide hydrolase; PPH beta; MEP1B elisa kit
Reactivity
Mouse
Specificity
The Mouse MEP1B ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse MEP1B proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
704
Samples
Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates
Sensitivity
<10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MEP1B elisa kit
Principle of the Assay: The Mouse MEP1B ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse MEP1B in Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Mouse MEP1B coated on a 96-well plate. Standards and samples are pipetted into the wells and MEP1B present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse MEP1B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MEP1B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases. Both meprin subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Merpin A (composed of alpha subunits with or without beta subunits) and Merpin B (composed of beta subunits only). Although the two meprin subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity.
Background: Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases. Both meprin subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Merpin A (composed of alpha subunits with or without beta subunits) and Merpin B (composed of beta subunits only). Although the two meprin subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,348 Da
NCBI Official Full Name
meprin A subunit beta
NCBI Official Synonym Full Names
meprin 1 beta
NCBI Official Symbol
Mep1b
NCBI Official Synonym Symbols
Mep-1b
NCBI Protein Information
meprin A subunit beta
UniProt Protein Name
Meprin A subunit beta
UniProt Gene Name
Mep1b
UniProt Synonym Gene Names
Mep-1b
UniProt Entry Name
MEP1B_MOUSE
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Product Notes
The Mouse MEP1B mep1b (Catalog #AAA263131) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263131 ELISA Kit recognizes Mouse MEP1B. It is sometimes possible for the material contained within the vial of "MEP1B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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