Mouse Meteorin/METRN ELISA Kit | METRN elisa kit

Mouse Meteorin/METRN PicoKine ELISA Kit

Cat. Num. AAA46780

• Gene Names: Metrn; Hyrac; 1810034B16Rik
• Reactivity: Mouse
• Synonyms: Meteorin/METRN; N/A; Mouse Meteorin/METRN PicoKine ELISA Kit; C16orf23; c380A1.2; Hyrac; Meteorin; Meteorin precursor; Metrn; Q8C1Q4 ; meteorin, glial cell differentiation regulator; METRN elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Meteorin
• Sequence Length: 291
• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml

• Anticoagulant: heparin or EDTA
• TMB incubation time: 15-20
• Immunogen: NSO, G22-D291
• Sample Data: Concentration(pg/ml): |0|62.5|125|250|500|1000|2000|4000; O.D.: |0.068|0.107|0.171|0.262|0.442|0.728|1.300|2.254
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |130|611|2300|152|685|2542; Standard Deviation: |6.11|33.61|144.90|10.49|52.75|213.53; CV(%): |4.7|5.5|6.3|6.9|7.7|8.4
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse Meteorin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Meteorin standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Mouse Meteorin/METRN PicoKine ELISA Kit standard curve)

Related Product Information for METRN elisa kit

For quantitative detection of mouse Meteorin in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Meteorin, glial cell differentiation regulator is a protein that in humans is encoded by the METRN gene. The mouse Metrn gene is mapped to chromosome 17. It is concluded that meteorin acts directly on satellite glial cells to induce a change in morphology and that transformed satellite glial cells induce the secondary effect of neurite extension. In cerebellar explants, meteorin induced conversion of cerebellar astrocytes into radial glia and promoted astrocyte differentiation from embryonic day 11.5 cerebral cortex neurospheres.
Principle: Mouse Meteorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for Meteorin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G22-D291) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Meteorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Meteorin amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 51317368
• NCBI GeneID: 70083
• NCBI Accession #: NP_598480.1
• NCBI GenBank Nucleotide #: NM_133719.2
• UniProt Accession #: Q8C1Q4; Q45G31; Q6P6N6; Q8CI64; Q922A8
• Molecular Weight: 13,797 Da
• NCBI Official Full Name: meteorin
• NCBI Official Synonym Full Names: meteorin, glial cell differentiation regulator
• NCBI Official Symbol: Metrn
• NCBI Official Synonym Symbols: Hyrac; 1810034B16Rik
• NCBI Protein Information: meteorin
• UniProt Protein Name: Meteorin
• UniProt Gene Name: Metrn
• UniProt Synonym Gene Names: Hyrac