Mouse Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit | MFGE8 elisa kit

Mouse Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit

Cat. Num. AAA137694

• Gene Names: mfge8a; mfge8l; im:6895406; zgc:110846
• Reactivity: Mouse
• Synonyms: Milk Fat Globule EGF Factor 8 (MFGE8); N/A; Mouse Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit; HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8; MFGE8 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Milk Fat Globule EGF Factor 8 (MFGE8).; No significant cross-reactivity or interference between Milk Fat Globule EGF Factor 8 (MFGE8) and analogues was observed.
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and other biological fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: 5.8pg/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for MFGE8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Milk Fat Globule EGF Factor 8 (MFGE8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Milk Fat Globule EGF Factor 8 (MFGE8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MFGE8 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 113674039
• NCBI GeneID: 541486
• NCBI Accession #: NP_001038241.1
• NCBI GenBank Nucleotide #: NM_001044776.1
• NCBI Official Synonym Full Names: milk fat globule-EGF factor 8 protein a
• NCBI Official Symbol: mfge8a
• NCBI Official Synonym Symbols: mfge8l; im:6895406; zgc:110846
• NCBI Protein Information: milk fat globule-EGF factor 8 protein a