Standard Curve (Sample)
(Fig.1. Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) Standard Curve.)
Mouse Mitochondrial Import Inner Membrane Translocase Subunit TIM44 (TIMM44) ELISA Kit | TIMM44 elisa kit
Mouse Mitochondrial Import Inner Membrane Translocase Subunit TIM44 (TIMM44) ELISA Kit
Gene Names
Timm44; Tim44; Mimt44; D8Ertd118e; 0710005E20Rik
Reactivity
Mouse
Synonyms
Mitochondrial Import Inner Membrane Translocase Subunit TIM44 (TIMM44); N/A; Mouse Mitochondrial Import Inner Membrane Translocase Subunit TIM44 (TIMM44) ELISA Kit; DKFZp686H05241; TIM44; mitochondrial inner membrane translocase; translocase of inner mitochondrial membrane 44; TIMM44 elisa kit
Reactivity
Mouse
Specificity
Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIMM44. No significant cross-reactivity or interference between Mouse TIMM44 and analogues was observed.
Sequence Length
452
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) Standard Curve.)
Related Product Information for TIMM44 elisa kit
Description: This Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMM44 in samples. An antibody specific for TIMM44 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMM44 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMM44 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMM44 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TIM44, a peripheral inner membrane protein, tethers mitochondrial HSP70 to the import channel. A model peptide substrate rapidly released mitochondrial HSP70 from TIM44, even in the absence of ATP hydrolysis. In vivo, the analogous interaction of translocating polypeptide would release mitochondrial HSP70 from the channel. TIMM44 is hydrophilic and contains no hydrophobic membrane-spanning segments. The N terminus has a coiled-coil motif and a matrix-targeting segment with 6 positively and no negatively charged residues. Western blot analysis showed that TIMM44 is present in membranes as well as matrix and is most likely a 45-kD peripheral membrane protein loosely associated with the inner membrane of mitochondria.
Background: TIM44, a peripheral inner membrane protein, tethers mitochondrial HSP70 to the import channel. A model peptide substrate rapidly released mitochondrial HSP70 from TIM44, even in the absence of ATP hydrolysis. In vivo, the analogous interaction of translocating polypeptide would release mitochondrial HSP70 from the channel. TIMM44 is hydrophilic and contains no hydrophobic membrane-spanning segments. The N terminus has a coiled-coil motif and a matrix-targeting segment with 6 positively and no negatively charged residues. Western blot analysis showed that TIMM44 is present in membranes as well as matrix and is most likely a 45-kD peripheral membrane protein loosely associated with the inner membrane of mitochondria.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitochondrial import inner membrane translocase subunit TIM44
NCBI Official Synonym Full Names
translocase of inner mitochondrial membrane 44
NCBI Official Symbol
Timm44
NCBI Official Synonym Symbols
Tim44; Mimt44; D8Ertd118e; 0710005E20Rik
NCBI Protein Information
mitochondrial import inner membrane translocase subunit TIM44
UniProt Protein Name
Mitochondrial import inner membrane translocase subunit TIM44
UniProt Gene Name
Timm44
UniProt Synonym Gene Names
Mimt44; Tim44
UniProt Entry Name
TIM44_MOUSE
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Product Notes
The Mouse TIMM44 timm44 (Catalog #AAA332240) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332240 ELISA Kit recognizes Mouse TIMM44. It is sometimes possible for the material contained within the vial of "Mitochondrial Import Inner Membrane Translocase Subunit TIM44 (TIMM44), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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