Mouse Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ELISA Kit | MOTS-c elisa kit
Mouse Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ELISA Kit
Reactivity
Mouse
Synonyms
Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c); N/A; Mouse Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ELISA Kit; MOTS-c elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of MOTS-c. No significant cross-reactivity or interference between MOTS-c and analogues was observed.
Assay Type
Quantitative Competitive
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
246.9-20,000pg/mL
Sensitivity
< 95.1pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MOTS-c were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MOTS-c were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for MOTS-c elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to MOTS-c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled MOTS-c and unlabeled MOTS-c (Standards or samples) with the pre-coated antibody specific to MOTS-c. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of MOTS-c in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of MOTS-c in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MOTS-c in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MOTS-c in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Product Categories/Family for MOTS-c elisa kit
References
Mitohormesis in Hypothalamic POMC Neurons Mediates Regular Exercise-Induced High-Turnover Metabolism; Cell Metab; 2021 Feb 2;33(2):334-349.e6.; PMID: 33535098; Gil Myoung Kang; Asan Institute for Life Science, University of Ulsan College of Medicine, Seoul 05505, Korea.
