Mouse Neuroendocrine secretory protein 55 (GNAS) ELISA Kit | GNAS elisa kit

Mouse Neuroendocrine secretory protein 55 (GNAS) ELISA Kit

Cat. Num. AAA246477

• Gene Names: GNAS; AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45
• Reactivity: Mouse
• Synonyms: Neuroendocrine secretory protein 55 (GNAS); N/A; Mouse Neuroendocrine secretory protein 55 (GNAS) ELISA Kit; GNAS elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of NESP55. No significant cross-reactivity or interference between NESP55 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NESP55 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GNAS elisa kit

Principle of the Assay: NESP55 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NESP55 antibody and an NESP55-HRP conjugate. The assay sample and buffer are incubated together with NESP55-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NESP55 concentration since NESP55 from samples and NESP55-HRP conjugate compete for the anti-NESP55 antibody binding site. Since the number of sites is limited, as more sites are occupied by NESP55 from the sample, fewer sites are left to bind NESP55-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NESP55 concentration in each sample is interpolated from this standard curve.

Intended Uses: This NESP55 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NESP55. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for GNAS elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 7532780
• NCBI GeneID: 2778
• Molecular Weight: 111,025 Da
• NCBI Official Full Name: neuroendocrine secretory protein 55
• NCBI Official Synonym Full Names: GNAS complex locus
• NCBI Official Symbol: GNAS
• NCBI Official Synonym Symbols: AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45
• NCBI Protein Information: protein ALEX; protein GNAS; protein NESP55; secretogranin VI; extra large alphas protein; neuroendocrine secretory protein; guanine nucleotide regulatory protein; alternative gene product encoded by XL-exon; adenylate cyclase-stimulating G alpha protein; guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1
• UniProt Protein Name: Neuroendocrine secretory protein 55
• UniProt Gene Name: GNAS
• UniProt Synonym Gene Names: GNAS1; NESP55
• UniProt Entry Name: GNAS3_HUMAN