Standard Curve (Sample)
Mouse NOS2/iNOS ELISA Kit | NOS2/iNOS elisa kit
Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit
Gene Names
NOS2; NOS; INOS; NOS2A; HEP-NOS
Reactivity
Mouse
Synonyms
NOS2/iNOS; N/A; Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit; NOS2/iNOS elisa kit
Reactivity
Mouse
Specificity
This kit recognizes Mouse NF-kappaB p65 in samples. No significant cross-reactivity or interference between Mouse NF-kappaB p65 and analogues was observed.
Sequence Length
1153
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.31-20ng/mL
Sensitivity
0.19ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NF-kappaB p65 were tested 20 times on one plate, respectively
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NF-kappaB p65 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NOS2/iNOS elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse NF-kappaB p65 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NF-kappaB p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NF-kappaB p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NF-kappaB p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse NF-kappaB p65. You can calculate the concentration of Mouse NF-kappaB p65 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NF-kappaB p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NF-kappaB p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NF-kappaB p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse NF-kappaB p65. You can calculate the concentration of Mouse NF-kappaB p65 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126,749 Da
NCBI Official Full Name
nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2, inducible
NCBI Official Symbol
NOS2
NCBI Official Synonym Symbols
NOS; INOS; NOS2A; HEP-NOS
NCBI Protein Information
nitric oxide synthase, inducible; NOS type II; NOS, type II; hepatocyte NOS; inducible NO synthase; inducible NOS; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Gene Name
NOS2
UniProt Synonym Gene Names
NOS2A; HEP-NOS; Inducible NOS; iNOS
UniProt Entry Name
NOS2_HUMAN
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Product Notes
The Mouse NOS2/iNOS nos2 (Catalog #AAA165581) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA165581 ELISA Kit recognizes Mouse NOS2/iNOS. It is sometimes possible for the material contained within the vial of "NOS2/iNOS, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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