Mouse Oncostatin M/OSM PicoKine ELISA Kit | Osm elisa kit

Mouse Oncostatin M/OSM PicoKine ELISA Kit

Cat. Num. AAA46158

• Gene Names: Osm; OncoM
• Reactivity: Mouse
• Synonyms: Oncostatin M/OSM PicoKine; N/A; Mouse Oncostatin M/OSM PicoKine ELISA Kit; Oncostatin-M; MGC20461; ONCM_HUMAN; Oncostatin M precursor; OncostatinM; OSM; OTTMUSP00000005249; RP23-453B22.1; oncostatin M; Osm elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Oncostatin M
• Sequence Length: 263
• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse Oncostatin M in cell culture supernates, serum and plasma(heparin, EDTA).

• Immunogen: E Coli, A24-R206
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse Oncostatin M standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Oncostatin M standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|113|468|1221|126|471|1288; Standard deviation|4.86|22.00|69.60|10.46|41.92|117.21; CV(%)|4.3|4.7|5.7|8.3|8.9|9.1
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.031|0.074|0.157|0.305|0.587|1.032|1.901|2.369
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Mouse Oncostatin M/OSM PicoKine ELISA Kit standard curve)

Related Product Information for Osm elisa kit

Principle of the assay: Mouse Oncostatin M ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Oncostatin M has been precoated onto 96-well plates. Standards(E Coli, A24-R206) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Oncostatin M is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Oncostatin M amount of sample captured in plate.

Background: OSM(Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor(LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.

NCBI and Uniprot Product Information

• NCBI GI #: 61656173
• NCBI GeneID: 18413
• NCBI Accession #: NP_001013383.1
• NCBI GenBank Nucleotide #: NM_001013365.2
• UniProt Accession #: P53347; Q3U1Y5; Q5SPX6
• Molecular Weight: 30,114 Da
• NCBI Official Full Name: oncostatin-M
• NCBI Official Synonym Full Names: oncostatin M
• NCBI Official Symbol: Osm
• NCBI Official Synonym Symbols: OncoM
• NCBI Protein Information: oncostatin-M
• UniProt Protein Name: Oncostatin-M
• UniProt Gene Name: Osm
• UniProt Synonym Gene Names: OSM
• UniProt Entry Name: ONCM_MOUSE