Standard Curve (Sample)
Mouse PM20D1 ELISA Kit | PM20D1 elisa kit
Mouse PM20D1 Immunoassay Kit
Reactivity
Mouse
Synonyms
PM20D1; N/A; Mouse PM20D1 Immunoassay Kit; Mouse PM20D1; PM20D1 elisa kit
Reactivity
Mouse
Specificity
No cross reactivity or interference was observed when test with recombinant human PM20D1 protein.
Assay Type
Quantitative Sandwich
Samples
Serum or plasma samples
Sensitivity
0.156 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within one assay) One sample of known concentration were tested 8 times on same plate. C.V.: 3.8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) One sample of known concentration were tested in 8 separate assays. C.V.: 4.9%
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PM20D1 elisa kit
Background: PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In a UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.
Principle of the Assay: This assay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The microtiter plate is precoated with affinity purified polyclonal antibody against mouse PM20D1. Standards and samples are pipetted into the wells and any mouse PM20D1 present is bounded by the immobilized antibody. After washing away any unbound substances, a biotin-labelled polyclonal antibody against mouse PM20D1 is added to the wells. After washing step to remove any unbound reagents, Streptavidinhorseradish peroxidase (STP-HRP) conjugate is added. After the last wash step, a HRP substrate solution containing 3,3?,5,5?-Tetramethylbenzidine (TMB) is added, and color develops in proportion to the amount of mouse PM20D1 bound initially. Color reaction is stopped by 2M H2SO4 and the optical density of the wells are determined using a microtiter plate reader at 450nm. Since the increases in absorbance are directly proportional to the amount of captured mouse PM20D1, the unknown sample concentration can be calculated from the standard curve included in each assay.
Intended Uses: This Mouse PM20D1 ELISA kit is designed for the detection and quantitative measurement of mouse PM20D1 in serum or plasma samples.
Principle of the Assay: This assay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The microtiter plate is precoated with affinity purified polyclonal antibody against mouse PM20D1. Standards and samples are pipetted into the wells and any mouse PM20D1 present is bounded by the immobilized antibody. After washing away any unbound substances, a biotin-labelled polyclonal antibody against mouse PM20D1 is added to the wells. After washing step to remove any unbound reagents, Streptavidinhorseradish peroxidase (STP-HRP) conjugate is added. After the last wash step, a HRP substrate solution containing 3,3?,5,5?-Tetramethylbenzidine (TMB) is added, and color develops in proportion to the amount of mouse PM20D1 bound initially. Color reaction is stopped by 2M H2SO4 and the optical density of the wells are determined using a microtiter plate reader at 450nm. Since the increases in absorbance are directly proportional to the amount of captured mouse PM20D1, the unknown sample concentration can be calculated from the standard curve included in each assay.
Intended Uses: This Mouse PM20D1 ELISA kit is designed for the detection and quantitative measurement of mouse PM20D1 in serum or plasma samples.
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Product Notes
The Mouse PM20D1 (Catalog #AAA60563) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA60563 ELISA Kit recognizes Mouse PM20D1. It is sometimes possible for the material contained within the vial of "PM20D1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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