Standard Curve (Sample)
(Fig.1. Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) Standard Curve.)
Mouse Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2) ELISA Kit | PLOD2 elisa kit
Mouse Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2) ELISA Kit
Gene Names
Plod2; LH2; Plod-2; D530025C14Rik
Reactivity
Mouse
Synonyms
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2); N/A; Mouse Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2) ELISA Kit; PLOD2; LH2; TLH; lysine hydroxylase 2; lysyl hydroxylase 2; telopeptide lysyl hydroxylase; PLOD2 elisa kit
Reactivity
Mouse
Specificity
Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLOD2. No significant cross-reactivity or interference between Mouse PLOD2 and analogues was observed.
Sequence Length
758
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) Standard Curve.)
Related Product Information for PLOD2 elisa kit
Description: This Mouse Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) ELISA Kit employs a two-site sandwich ELISA to quantitate PLOD2 in samples. An antibody specific for PLOD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLOD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLOD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLOD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PLOD2 is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. The similarity is even higher in the C-terminal region of the polypeptides. The PLOD2 gene contains 9 cysteines conserved in PLOD, in addition to conserved histidines in the putative active site.
Background: PLOD2 is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. The similarity is even higher in the C-terminal region of the polypeptides. The PLOD2 gene contains 9 cysteines conserved in PLOD, in addition to conserved histidines in the putative active site.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 isoform 1
NCBI Official Synonym Full Names
procollagen lysine, 2-oxoglutarate 5-dioxygenase 2
NCBI Official Symbol
Plod2
NCBI Official Synonym Symbols
LH2; Plod-2; D530025C14Rik
NCBI Protein Information
procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
UniProt Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
UniProt Gene Name
Plod2
UniProt Synonym Gene Names
LH2
UniProt Entry Name
PLOD2_MOUSE
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Product Notes
The Mouse PLOD2 plod2 (Catalog #AAA332402) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332402 ELISA Kit recognizes Mouse PLOD2. It is sometimes possible for the material contained within the vial of "Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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