Mouse Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12) ELISA Kit | P2ry12 elisa kit

Mouse Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12) ELISA Kit

Cat. Num. AAA219801

• Gene Names: P2ry12; P2Y12; 2900079B22Rik; 4921504D23Rik
• Reactivity: Mouse
• Synonyms: Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12); N/A; Mouse Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12) ELISA Kit; ADPG-R; HORK3; P2T(AC); P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999; ADP-glucose receptor; P2Y12 platelet ADP receptor; P2ry12 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of P2RY12. No significant cross-reactivity or interference between P2RY12 and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Mouse tissue homogenates, cell lysates or other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level P2RY12 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level P2RY12 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Shelf Life: 12 months

Standard Curve (Sample)

Related Product Information for P2ry12 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P2RY12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to P2RY12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P2RY12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of P2RY12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of P2RY12 in mouse tissue homogenates, cell lysates or other biological fluids.

Product Categories/Family for P2ry12 elisa kit

Traditional ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 169790983
• NCBI GeneID: 70839
• NCBI Accession #: NP_081847.3
• NCBI GenBank Nucleotide #: NM_027571.3
• UniProt Accession #: Q9CPV9
• Molecular Weight: 39,474 Da
• NCBI Official Full Name: P2Y purinoceptor 12
• NCBI Official Synonym Full Names: purinergic receptor P2Y, G-protein coupled 12
• NCBI Official Symbol: P2ry12
• NCBI Official Synonym Symbols: P2Y12; 2900079B22Rik; 4921504D23Rik
• NCBI Protein Information: P2Y purinoceptor 12
• UniProt Protein Name: P2Y purinoceptor 12
• UniProt Gene Name: P2ry12
• UniProt Synonym Gene Names: P2Y12
• UniProt Entry Name: P2Y12_MOUSE