Standard Curve (Sample)
(Mouse Sclerostin/SOST PicoKine ELISA Kit standard curve)
Mouse Sclerostin/SOST ELISA Kit | SOST elisa kit
Mouse Sclerostin/SOST PicoKine ELISA Kit
Gene Names
Sost; 5430411E23Rik
Reactivity
Mouse
Synonyms
Sclerostin/SOST; N/A; Mouse Sclerostin/SOST PicoKine ELISA Kit; Sclerostin; sclerostin; BEER; CDD; Cortical hyperostosis with syndactyly; Sclerosteosis; SOST; SOST_HUMAN; UNQ2976/PRO7455/PRO7476; VBCH; SOST elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse Sclerostin
Sequence Length
211
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse Sclerostin/SOST PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse Sclerostin/SOST PicoKine ELISA Kit standard curve)
Related Product Information for SOST elisa kit
Principle of the assay: mouse Sclerostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Sclerostin has been precoated onto 96-well plates. Standards(NSO, Q24-Y211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Sclerostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Sclerostin amount of sample captured in plate.
Background: Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21
Background: Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,443 Da
NCBI Official Full Name
Sclerostin
NCBI Official Synonym Full Names
sclerostin
NCBI Official Symbol
Sost
NCBI Official Synonym Symbols
5430411E23Rik
NCBI Protein Information
sclerostin
UniProt Protein Name
Sclerostin
UniProt Gene Name
Sost
UniProt Entry Name
SOST_MOUSE
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Product Notes
The Mouse SOST sost (Catalog #AAA45974) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45974 ELISA Kit recognizes Mouse SOST. It is sometimes possible for the material contained within the vial of "Sclerostin/SOST, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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