Mouse Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit | SFRP1 elisa kit

Mouse Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

Cat. Num. AAA17291

• Gene Names: Sfrp1; sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik
• Reactivity: Mouse
• Synonyms: Secreted Frizzled Related Protein 1 (SFRP1); N/A; Mouse Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit; SFRP1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SFRP1. No significant cross-reactivity or interference between SFRP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SFRP1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for SFRP1 elisa kit

Principle of the Assay: SFRP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SFRP1 antibody and an SFRP1-HRP conjugate. The assay sample and buffer are incubated together with SFRP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SFRP1 concentration since SFRP1 from samples and SFRP1-HRP conjugate compete for the anti-SFRP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SFRP1 from the sample, fewer sites are left to bind SFRP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SFRP1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This SFRP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SFRP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 65301472
• NCBI GeneID: 20377
• NCBI Accession #: NP_038862.2
• NCBI GenBank Nucleotide #: NM_013834.3
• UniProt Accession #: Q8C4U3; O08861; Q505A2; Q8R1J4
• Molecular Weight: 35,413 Da
• NCBI Official Full Name: secreted frizzled-related protein 1
• NCBI Official Synonym Full Names: secreted frizzled-related protein 1
• NCBI Official Symbol: Sfrp1
• NCBI Official Synonym Symbols: sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik
• NCBI Protein Information: secreted frizzled-related protein 1; secreted frizzled-related sequence protein 1
• UniProt Protein Name: Secreted frizzled-related protein 1
• UniProt Gene Name: Sfrp1
• UniProt Synonym Gene Names: sFRP-1
• UniProt Entry Name: SFRP1_MOUSE