Standard Curve (Sample)
(Fig.1. Mouse Serine palmitoyltransferase 3 (SPTLC3) Standard Curve.)
Mouse Serine Palmitoyltransferase 3 (SPTLC3) ELISA Kit | SPTLC3 elisa kit
Mouse Serine Palmitoyltransferase 3 (SPTLC3) ELISA Kit
Gene Names
Sptlc3; C130053K05Rik
Reactivity
Mouse
Synonyms
Serine Palmitoyltransferase 3 (SPTLC3); N/A; Mouse Serine Palmitoyltransferase 3 (SPTLC3) ELISA Kit; SPTLC3; RP5-1077I2.1; C20orf38; FLJ11112; FLJ90790; LCB3; SPT3; SPTLC2L; dJ718P11; dJ718P11.1; long chain base biosynthesis protein 3; serine palmitoyltransferase; long chain base subunit 2-like (aminotransf; SPTLC3 elisa kit
Reactivity
Mouse
Specificity
Mouse Serine palmitoyltransferase 3 (SPTLC3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPTLC3. No significant cross-reactivity or interference between Mouse SPTLC3 and analogues was observed.
Sequence Length
563
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Serine palmitoyltransferase 3 (SPTLC3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Serine palmitoyltransferase 3 (SPTLC3) Standard Curve.)
Related Product Information for SPTLC3 elisa kit
Description: This Mouse Serine palmitoyltransferase 3 (SPTLC3) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTLC3 in samples. An antibody specific for SPTLC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPTLC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTLC3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTLC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPTLC3 orthologs were found in rat, mouse, and dog. Quantitative real-time PCR of human tissues showed highest expression in placenta, followed by skin, adrenal gland, testis, uterus, salivary gland, prostate, and kidney, lower expression in all other tissues, and no expression in peripheral blood cells and bone marrow. SPTLC3 and SPTLC2 showed different expression patterns, possibly reflecting tissue-specific requirements of sphingolipid synthesis. Western blot analysis revealed SPTLC3 expression in human trophoblast cell lines. Overexpression of SPTLC3 in HEK293 cells, which have little endogenous SPTLC3, resulted in increased SPT activity by 2- to 3-fold. Expression of an SPTLC3-specific siRNA in HepG2 cells and human trophoblast cells significantly decreased SPT activity, indicating that SPTLC3 plays a role in SPT activity.
Background: SPTLC3 orthologs were found in rat, mouse, and dog. Quantitative real-time PCR of human tissues showed highest expression in placenta, followed by skin, adrenal gland, testis, uterus, salivary gland, prostate, and kidney, lower expression in all other tissues, and no expression in peripheral blood cells and bone marrow. SPTLC3 and SPTLC2 showed different expression patterns, possibly reflecting tissue-specific requirements of sphingolipid synthesis. Western blot analysis revealed SPTLC3 expression in human trophoblast cell lines. Overexpression of SPTLC3 in HEK293 cells, which have little endogenous SPTLC3, resulted in increased SPT activity by 2- to 3-fold. Expression of an SPTLC3-specific siRNA in HepG2 cells and human trophoblast cells significantly decreased SPT activity, indicating that SPTLC3 plays a role in SPT activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine palmitoyltransferase 3
NCBI Official Synonym Full Names
serine palmitoyltransferase, long chain base subunit 3
NCBI Official Symbol
Sptlc3
NCBI Official Synonym Symbols
C130053K05Rik
NCBI Protein Information
serine palmitoyltransferase 3
UniProt Protein Name
Serine palmitoyltransferase 3
UniProt Gene Name
Sptlc3
UniProt Synonym Gene Names
Sptlc2l; LCB2b; LCB 3; SPT 3
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Product Notes
The Mouse SPTLC3 sptlc3 (Catalog #AAA332264) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332264 ELISA Kit recognizes Mouse SPTLC3. It is sometimes possible for the material contained within the vial of "Serine Palmitoyltransferase 3 (SPTLC3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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