Mouse SFTPD/SP-D/SFTP4 ELISA Kit | SFTPD/SP-D/SFTP4 elisa kit

Mouse SFTPD/SP-D/SFTP4 ELISA Kit

Cat. Num. AAA258480

• Reactivity: Mouse
• Synonyms: SFTPD/SP-D/SFTP4; N/A; Mouse SFTPD/SP-D/SFTP4 ELISA Kit; Surfactant protein D/SFTPD ELISA Kit, Mouse; surfactant protein D; AI573415 ELISA Kit, Mouse; Sftp4 ELISA Kit, Mouse; SP-D ELISA Kit, Mouse; SFTPD/SP-D/SFTP4 elisa kit

• Reactivity: Mouse
• Specificity: The kit has been verified by high purity Mouse SFTPD/SP-D/SFTP4 recombinant protein.
• Assay Type: Solid Phase Sandwich ELISA (quantitative)
• Sample Types: Recombinant protein
• Detection Range: 93.75-6000pg/mL
• Sensitivity: 48.95pg/mL
• Conjugation: HRP
• Materials Provided: 1. 96 well microplate coated with Capture Antibody; 2. Detection Antibody conjugated to HRP; 3. Standards; 4. Wash Buffer Concentrate; 5. Dilution Buffer Concentrate; 6. Color Reagent A; 7. Color Reagent B; 8. Stop Solution

Application Data

(This assay recognizes1 recombinant Mouse SFTPD. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)

Standard Curve (Sample)

(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)

Related Product Information for SFTPD/SP-D/SFTP4 elisa kit

This Surfactant protein D/SFTPD ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Surfactant protein D/SFTPD protein in Recombinant protein. It contains recombinant Mouse Surfactant protein D/SFTPD, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Background: Surfactant pulmonary-associated protein D, also known as SFTPD and SP-D, is a member of the collectin family of C-type lectins that is synthesized in many tissues including respiratory epithelial cells in the lung, and contains one C-type lectin domain and one collagen-like domain. The polymorphic variation in the N-terminal domain of the SP-D molecule influences oligomerization, function, and the concentration of the molecule in serum. SFTPD is produced primarily by alveolar type II cells and nonciliated bronchiolar cells in the lung and is constitutively secreted into the alveoli where it influences surfactant homeostasis, effector cell functions, and host defense. It is upregulated in a variety of inflammatory and infectious conditions including Pneumocystis pneumonia and asthma. SFTPD is humoral molecules of the innate immune system, and is considered a functional candidate in chronic periodontitis. Besides, it is involved in the development of acute and chronic inflammation of the lung. Several human lung diseases are characterized by decreased levels of bronchoalveolar SFTPD. Thus, recombinant SFTPD has been proposed as a therapeutical option for cystic fibrosis, neonatal lung disease and smoking-induced emphysema. Furthermore, SFTPD serum levels can be used as disease activity markers for interstitial lung diseases.

References

Leth-Larsen R, et al. (2005) A common polymorphism in the SFTPD gene influences assembly, function, and concentration of surfactant protein D. J Immunol. 174(3): 1532-8.
Moran AP, et al. (2005) Role of surfactant protein D (SP-D) in innate immunity in the gastric mucosa: evidence of interaction with Helicobacter pylori lipopolysaccharide. J Endotoxin Res. 11(6): 357-62.
Hartl D, et al. (2006) Surfactant protein D in human lung diseases. Eur J Clin Invest. 36(6): 423-35.
Krueger M, et al. (2006) Amino acid variants in Surfactant protein D are not associated with bronchial asthma. Pediatr Allergy Immunol. 17(1): 77-81.
Glas J, et al. (2008) Increased plasma concentration of surfactant protein D in chronic periodontitis independent of SFTPD genotype: potential role as a biomarker. Tissue Antigens. 72(1): 21-8.