Standard Curve (Sample)
(Fig.1. Mouse Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) Standard Curve.)
Mouse Soluble Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (sTRAIL) ELISA Kit | sTRAIL elisa kit
Mouse Soluble Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (sTRAIL) ELISA Kit
Gene Names
Tnfsf10; TL2; Ly81; Trail; APO-2L; Tnlg6a; AI448571; A330042I21Rik
Reactivity
Mouse
Synonyms
Soluble Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (sTRAIL); N/A; Mouse Soluble Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (sTRAIL) ELISA Kit; TNFSF10; APO2L; Apo-2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family; member 10; tumor necrosis factor apoptosis-inducing ligand splice var; sTRAIL elisa kit
Reactivity
Mouse
Specificity
Mouse Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF10. No significant cross-reactivity or interference between Mouse TNFSF10 and analogues was observed.
Sequence Length
291
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) Standard Curve.)
Related Product Information for sTRAIL elisa kit
Description: This Mouse Soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF10 in samples. An antibody specific for TNFSF10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFSF10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: This protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
Background: This protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 10
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 10
NCBI Official Symbol
Tnfsf10
NCBI Official Synonym Symbols
TL2; Ly81; Trail; APO-2L; Tnlg6a; AI448571; A330042I21Rik
NCBI Protein Information
tumor necrosis factor ligand superfamily member 10
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 10
UniProt Gene Name
Tnfsf10
UniProt Synonym Gene Names
Trail; Protein TRAIL
UniProt Entry Name
TNF10_MOUSE
Similar Products
Product Notes
The Mouse sTRAIL tnfsf10 (Catalog #AAA332132) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332132 ELISA Kit recognizes Mouse sTRAIL. It is sometimes possible for the material contained within the vial of "Soluble Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (sTRAIL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.