Mouse Syndecan-1/SDC1 ELISA Kit | Sdc1 elisa kit

Mouse Syndecan-1/SDC1 PicoKine ELISA Kit

Cat. Num. AAA11688

• Gene Names: Sdc1; Sstn; Synd; CD138; Synd1; syn-1; AA408134; AA409076
• Reactivity: Mouse
• Synonyms: Syndecan-1/SDC1; N/A; Mouse Syndecan-1/SDC1 PicoKine ELISA Kit; CD138; P18827; SDC1; SYND1; Synd-1; Synd1; syndecan1; Syndecan-1; Sdc1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Sdc1
• Sequence Length: 311
• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for Sdc1 elisa kit

Background: Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What's more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.

Principle of the Assay: The Picokine™ Mouse Sdc1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Sdc1 with a 96-well strip plate that is pre-coated with antibody specific for Sdc1. The detection antibody is a biotinylated antibody specific for Sdc1. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Sdc1 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q18-E252. The kit is analytically validated with ready-to-use reagents. To measure Mouse Sdc1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Sdc1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Sdc1 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 6755438
• NCBI GeneID: 20969
• NCBI Accession #: NP_035649.1
• NCBI GenBank Nucleotide #: NM_011519.2
• UniProt Accession #: P18828; Q62278; Q9WVD2
• Molecular Weight: 28,235 Da
• NCBI Official Full Name: syndecan-1
• NCBI Official Synonym Full Names: syndecan 1
• NCBI Official Symbol: Sdc1
• NCBI Official Synonym Symbols: Sstn; Synd; CD138; Synd1; syn-1; AA408134; AA409076
• NCBI Protein Information: syndecan-1
• UniProt Protein Name: Syndecan-1
• UniProt Gene Name: Sdc1
• UniProt Synonym Gene Names: Synd-1; Synd1; SYND1