Standard Curve (Sample)
(Fig.1. Mouse TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) Standard Curve.)
Mouse TCDD-Inducible Poly [ADP-Ribose] Polymerase (TIPARP) ELISA Kit | TIPARP elisa kit
Mouse TCDD-Inducible Poly [ADP-Ribose] Polymerase (TIPARP) ELISA Kit
Gene Names
Tiparp; PARP7; ARTD14; AW558171
Reactivity
Mouse
Synonyms
TCDD-Inducible Poly [ADP-Ribose] Polymerase (TIPARP); N/A; Mouse TCDD-Inducible Poly [ADP-Ribose] Polymerase (TIPARP) ELISA Kit; TIPARP; DDF1; DKFZp434J214; DKFZp686N0351; DKFZp686P1838; FLJ40466; PARP-1; PARP-7; PARP7; TIPARP elisa kit
Reactivity
Mouse
Specificity
Mouse TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIPARP. No significant cross-reactivity or interference between Mouse TIPARP and analogues was observed.
Sequence Length
657
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) Standard Curve.)
Related Product Information for TIPARP elisa kit
Description: This Mouse TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPARP in samples. An antibody specific for TIPARP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIPARP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPARP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPARP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TIPARP, encodes a deduced 657-amino acid protein that shares 91.8% and 52.7% sequence identity with mouse and fugu Tiparp proteins, respectively. The proteins in all 3 species contain 3 highly conserved domains: a WWE (trp, trp, glu) domain, a PARP (poly(ADP-ribose) polymerase)-like catalytic domain, and a novel TPH (TIPARP homologous) domain. The N-terminal part of the TPH domain contains a CCCH-type zinc finger. Katoh and Katoh (2003) noted that the deduced proteins of 2 human genes, which they called FLJ226093 (PARP12) and ZAP (ZC3HAV1), contain the same 3 conserved domains and share 27.5% and 26% overall sequence identity with TIPARP, respectively.
Background: TIPARP, encodes a deduced 657-amino acid protein that shares 91.8% and 52.7% sequence identity with mouse and fugu Tiparp proteins, respectively. The proteins in all 3 species contain 3 highly conserved domains: a WWE (trp, trp, glu) domain, a PARP (poly(ADP-ribose) polymerase)-like catalytic domain, and a novel TPH (TIPARP homologous) domain. The N-terminal part of the TPH domain contains a CCCH-type zinc finger. Katoh and Katoh (2003) noted that the deduced proteins of 2 human genes, which they called FLJ226093 (PARP12) and ZAP (ZC3HAV1), contain the same 3 conserved domains and share 27.5% and 26% overall sequence identity with TIPARP, respectively.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein mono-ADP-ribosyltransferase TIPARP
NCBI Official Synonym Full Names
TCDD-inducible poly(ADP-ribose) polymerase
NCBI Official Symbol
Tiparp
NCBI Official Synonym Symbols
PARP7; ARTD14; AW558171
NCBI Protein Information
protein mono-ADP-ribosyltransferase TIPARP
UniProt Protein Name
TCDD-inducible poly [ADP-ribose] polymerase
UniProt Gene Name
Tiparp
UniProt Synonym Gene Names
ARTD14
UniProt Entry Name
PARPT_MOUSE
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Product Notes
The Mouse TIPARP tiparp (Catalog #AAA332235) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332235 ELISA Kit recognizes Mouse TIPARP. It is sometimes possible for the material contained within the vial of "TCDD-Inducible Poly [ADP-Ribose] Polymerase (TIPARP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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