Mouse TGFbeta2 ELISA Kit | TGFbeta2 elisa kit
TGFbeta2 Mouse ELISA Kit
Gene Names
TGFB2; LDS4; TGF-beta2
Reactivity
Mouse
Applications
ELISA
Synonyms
TGFbeta2; N/A; TGFbeta2 Mouse ELISA Kit; Mouse TGFbeta2; TGFbeta2 elisa kit
Reactivity
Mouse
Assay Type
Sandwich
Detection Range
10pg/ml-800 pg/ml
Applicable Applications for TGFbeta2 elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of TGF-beta2 in mouse serum, plasma, urine, cell culture supernatant or tissue samples.
Preparation and Storage
Store at 2-8 degree C for 6 months.
Standard Curve (Sample)
Related Product Information for TGFbeta2 elisa kit
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-TGF- beta 2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-TGF-beta2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGF-beta2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGF-beta2 can be calculated.
Background: Transforming growth factor-beta 2 (TGF-beta2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is known to suppress the effects of interleukin dependent T-cell tumors. It is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma. In 2004, Gottanka found that TGF-beta2 reduced outflow facility when perfused into cultured Mouse anterior segments. Furthermore, TGF-beta2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility.
Background: Transforming growth factor-beta 2 (TGF-beta2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is known to suppress the effects of interleukin dependent T-cell tumors. It is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma. In 2004, Gottanka found that TGF-beta2 reduced outflow facility when perfused into cultured Mouse anterior segments. Furthermore, TGF-beta2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,748 Da
NCBI Official Full Name
transforming growth factor beta-2 isoform 1
NCBI Official Synonym Full Names
transforming growth factor, beta 2
NCBI Official Symbol
TGFB2
NCBI Official Synonym Symbols
LDS4; TGF-beta2
NCBI Protein Information
transforming growth factor beta-2; G-TSF; cetermin; polyergin; BSC-1 cell growth inhibitor; glioblastoma-derived T-cell suppressor factor
UniProt Protein Name
Transforming growth factor beta-2
UniProt Gene Name
TGFB2
UniProt Synonym Gene Names
TGF-beta-2; G-TSF; LAP
UniProt Entry Name
TGFB2_HUMAN
