Standard Curve (Sample)
(Fig.1. Mouse Thioredoxin reductas (TRXR) Standard Curve.)
Mouse Thioredoxin reductas (TRXR) ELISA Kit | TRXR elisa kit
Mouse Thioredoxin reductas (TRXR) ELISA Kit
Gene Names
Txnrd1; TR; TR1; TrxR1
Reactivity
Mouse
Synonyms
Thioredoxin reductas (TRXR); N/A; Mouse Thioredoxin reductas (TRXR) ELISA Kit; TXNRD1; GRIM-12; MGC9145; TR; TR1; TRXR1; TXNR; KM-102-derived reductase-like factor; oxidoreductase; thioredoxin reductase GRIM-12; TRXR elisa kit
Reactivity
Mouse
Specificity
Mouse Thioredoxin reductas (TRXR) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TXNRD1. No significant cross-reactivity or interference between Mouse TXNRD1 and analogues was observed.
Sequence Length
499
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Thioredoxin reductas (TRXR) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Thioredoxin reductas (TRXR) Standard Curve.)
Related Product Information for TRXR elisa kit
Description: This Mouse Thioredoxin reductas (TRXR) ELISA Kit employs a two-site sandwich ELISA to quantitate TXNRD1 in samples. An antibody specific for TXNRD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TXNRD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXNRD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXNRD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thioredoxin reductase 1, cytoplasmic is a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
Background: Thioredoxin reductase 1, cytoplasmic is a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thioredoxin reductase 1, cytoplasmic isoform 1
NCBI Official Synonym Full Names
thioredoxin reductase 1
NCBI Official Symbol
Txnrd1
NCBI Official Synonym Symbols
TR; TR1; TrxR1
NCBI Protein Information
thioredoxin reductase 1, cytoplasmic
UniProt Protein Name
Thioredoxin reductase 1, cytoplasmic
UniProt Gene Name
Txnrd1
UniProt Synonym Gene Names
Trxr1; TR
UniProt Entry Name
TRXR1_MOUSE
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Product Notes
The Mouse TRXR txnrd1 (Catalog #AAA332058) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332058 ELISA Kit recognizes Mouse TRXR. It is sometimes possible for the material contained within the vial of "Thioredoxin reductas (TRXR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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