Standard Curve (Sample)
Mouse Thrombopoietin / TPO ELISA Kit | TPO elisa kit
Thrombopoietin / TPO Mouse ELISA Kit
Gene Names
THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
Reactivity
Mouse
Applications
ELISA
Synonyms
Thrombopoietin / TPO; N/A; Thrombopoietin / TPO Mouse ELISA Kit; Mouse Thrombopoietin / TPO; TPO elisa kit
Reactivity
Mouse
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
< 5pg/ml
Applicable Applications for TPO elisa kit
ELISA
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TPO elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TPO polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TPO polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TPO amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TPO can be calculated.
Background: Thrombopoietin (THPO) also known as megakaryocyte growth and development factor (MGDF) is a protein that in humans is encoded by the THPO gene. The gene is 6.2 kb long and contains 6 exons and 5 introns, and maps to 3q27. THPO was expressed from both promoters in HEK293T human embryonic kidney cells and in HepG2 adult liver cells. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene.
Background: Thrombopoietin (THPO) also known as megakaryocyte growth and development factor (MGDF) is a protein that in humans is encoded by the THPO gene. The gene is 6.2 kb long and contains 6 exons and 5 introns, and maps to 3q27. THPO was expressed from both promoters in HEK293T human embryonic kidney cells and in HepG2 adult liver cells. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,823 Da
NCBI Official Full Name
thrombopoietin
NCBI Official Synonym Full Names
thrombopoietin
NCBI Official Symbol
THPO
NCBI Official Synonym Symbols
ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
NCBI Protein Information
thrombopoietin; MPL ligand; c-mpl ligand; thrombopoietin nirs variant 1; megakaryocyte stimulating factor; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand
UniProt Protein Name
Thrombopoietin
UniProt Gene Name
THPO
UniProt Synonym Gene Names
MGDF; ML; MGDF
UniProt Entry Name
TPO_HUMAN
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Product Notes
The Mouse TPO thpo (Catalog #AAA59005) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA59005 ELISA Kit recognizes Mouse TPO. AAA Biotech's Thrombopoietin / TPO can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TPO thpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thrombopoietin / TPO, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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