Standard Curve (Sample)
Mouse Thymosin beta-10 (TMSB10) ELISA Kit | TMSB10 elisa kit
Mouse Thymosin beta-10 (TMSB10) ELISA Kit
Gene Names
Tmsb10; Tb10; Ptmb10
Reactivity
Mouse
Synonyms
Thymosin beta-10 (TMSB10); N/A; Mouse Thymosin beta-10 (TMSB10) ELISA Kit; TMSB10; MIG12; TB10; migration-inducing gene 12; migration-inducing protein 12; thymosin; beta 10; TMSB10 elisa kit
Reactivity
Mouse
Specificity
Mouse Thymosin beta-10 (TMSB10) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMSB10. No significant cross-reactivity or interference between Mouse TMSB10 and analogues was observed.
Sequence Length
44
Assay Type
Sandwich
Samples
Mouse serum, cell culture supernates and other biological fluids
Detection Range
20 pg/ml - 320 pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Four samples of known concentration were tested tw enty times o n one plate to assess intraintra-assay prec ision. The C V (%) (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested i n twenty separate assays to assess interinter-assay pre cision. Assa ys were per formed by at least three t echnicians using two lots of compon ents. The CV (%) ?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TMSB10 elisa kit
Background: Thymosin-beta-10 is closely related in sequence to TMSB4 and is also an actin-sequestering protein. In most tissues of the rat, the same molecular weight species of TMSB10 mRNA is found; This finding, together with primer extension experiments, suggested that the 2 mRNAs are transcribed from the same gene through a combination of differential promoter utilization and alternative splicing. Both mRNAs were present in pachytene spermatocytes; only the testis-specific mRNA was detected in postmeiotic haploid spermatids. Immunohistochemical analysis showed that the protein was present in differentiating spermatids, suggesting that the testis-specific TMSB10 mRNA is translated in haploid male germ cells. thymosin-beta-10 protein synthesized in adult testis was identical in size to that synthesized in brain.
Principle of the Assay: Mouse Thymosin beta-10 (TMSB10) ELISA Kit employs a two-site sandwich ELISA to quantitate TMSB10 in samples. An antibody specific for TMSB10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMSB10 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse TMSB10 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TMSB10 bound in the initial step.
Principle of the Assay: Mouse Thymosin beta-10 (TMSB10) ELISA Kit employs a two-site sandwich ELISA to quantitate TMSB10 in samples. An antibody specific for TMSB10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMSB10 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Mouse TMSB10 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TMSB10 bound in the initial step.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thymosin beta-10
NCBI Official Synonym Full Names
thymosin, beta 10
NCBI Official Symbol
Tmsb10
NCBI Official Synonym Symbols
Tb10; Ptmb10
NCBI Protein Information
thymosin beta-10
UniProt Protein Name
Thymosin beta-10
UniProt Gene Name
Tmsb10
UniProt Synonym Gene Names
Ptmb10
UniProt Entry Name
TYB10_MOUSE
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Product Notes
The Mouse TMSB10 tmsb10 (Catalog #AAA332160) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332160 ELISA Kit recognizes Mouse TMSB10. It is sometimes possible for the material contained within the vial of "Thymosin beta-10 (TMSB10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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