Mouse Thyroid Stimulating Hormone (TSH) ELISA Kit | TSH elisa kit

Mouse Thyroid Stimulating Hormone (TSH) ELISA Kit

Cat. Num. AAA22803

• Reactivity: Mouse
• Synonyms: Thyroid Stimulating Hormone (TSH); Mouse Thyroid Stimulating Hormone (TSH) ELISA Kit; Thyrotropin; TSH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TSH. No significant cross-reactivity or interference between TSH and analogues was observed.
• Assay Type: Quantitative Competitive
• Samples: Mouse serum, plasma and other biological fluids
• Detection Range: 6.4-4,000pg/mL
• Sensitivity: < 2.5pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TSH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TSH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for TSH elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TSH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled TSH and unlabeled TSH (Standards or samples) with the pre-coated antibody specific to TSH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of TSH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of TSH in the sample.

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of TSH in mouse serum, plasma and other biological fluids.