Standard Curve (Sample)
Mouse TIMP4 ELISA Kit | TIMP4 elisa kit
Mouse TIMP4 ELISA Kit
Reactivity
Mouse
Applications
ELISA
Synonyms
TIMP4; N/A; Mouse TIMP4 ELISA Kit; Metalloproteinase inhibitor 4; Tissue inhibitor of metalloproteinases 4; TIMP-4; TIMP4 elisa kit
Reactivity
Mouse
Specificity
The Mouse TIMP4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse TIMP4 proteins within the range of 78 pg/ml - 5000 pg/ml.
Sequence Length
224
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Applicable Applications for TIMP4 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TIMP4 elisa kit
Principle of the Assay||The Mouse TIMP4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TIMP4 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse TIMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TIMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What's more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.
Background/Introduction: Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What's more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,503 Da
NCBI Official Full Name
Metalloproteinase inhibitor 4
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 4
NCBI Official Symbol
TIMP4
NCBI Protein Information
metalloproteinase inhibitor 4
UniProt Protein Name
Metalloproteinase inhibitor 4
UniProt Gene Name
TIMP4
UniProt Synonym Gene Names
TIMP-4
UniProt Entry Name
TIMP4_HUMAN
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Product Notes
The Mouse TIMP4 timp4 (Catalog #AAA261653) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261653 ELISA Kit recognizes Mouse TIMP4. AAA Biotech's TIMP4 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the TIMP4 timp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIMP4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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