Standard Curve (Sample)
(Fig.1. Mouse Trace amine-associated receptor 2 (TAAR2) Standard Curve.)
Mouse Trace Amine-Associated Receptor 2 (TAAR2) ELISA Kit | TAAR2 elisa kit
Mouse Trace Amine-Associated Receptor 2 (TAAR2) ELISA Kit
Gene Names
Taar2; Gm224; Gm225; Gpr58; Tarr2
Reactivity
Mouse
Synonyms
Trace Amine-Associated Receptor 2 (TAAR2); N/A; Mouse Trace Amine-Associated Receptor 2 (TAAR2) ELISA Kit; TAAR2; GPR58; G protein-coupled receptor 58; OTTHUMP00000017225; TAAR2 elisa kit
Reactivity
Mouse
Specificity
Mouse Trace amine-associated receptor 2 (TAAR2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAAR2. No significant cross-reactivity or interference between Mouse TAAR2 and analogues was observed.
Sequence Length
339
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Trace amine-associated receptor 2 (TAAR2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Trace amine-associated receptor 2 (TAAR2) Standard Curve.)
Related Product Information for TAAR2 elisa kit
Description: This Mouse Trace amine-associated receptor 2 (TAAR2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAAR2 in samples. An antibody specific for TAAR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAAR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAAR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAAR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Trace amine-associated receptor 2 (TAAR2), formerly known as G protein-coupled receptor 58 (GPR58), is a protein that in humans is encoded by the TAAR2 gene. TAAR2 is coexpressed with Galpha proteins; however, as of February 2017, its signal transduction mechanisms have not been determined. Human TAAR2 (hTAAR2) is expressed in the cerebellum, olfactory sensory neurons in the olfactory epithelium, and leukocytes (i.e., white blood cells), among other tissues.[7][8] hTAAR1 and hTAAR2 are both required for white blood cell activation by trace amines in granulocytes.[9] Phenethylamine, para-tyramine, and 3-iodothyronamine are full agonists of hTAAR2 with EC50 values of 0.43 +/- 0.05 nM, 0.52 +/- 0.05 nM, and 0.25 +/- 0.04 nM respectively. A single nucleotide polymorphism nonsense mutation of the TAAR2 gene is associated with schizophrenia. TAAR2 is a probable pseudogene in 10-15% of Asians as a result of a polymorphism that produces a premature stop codon at amino acid 168.
Background: Trace amine-associated receptor 2 (TAAR2), formerly known as G protein-coupled receptor 58 (GPR58), is a protein that in humans is encoded by the TAAR2 gene. TAAR2 is coexpressed with Galpha proteins; however, as of February 2017, its signal transduction mechanisms have not been determined. Human TAAR2 (hTAAR2) is expressed in the cerebellum, olfactory sensory neurons in the olfactory epithelium, and leukocytes (i.e., white blood cells), among other tissues.[7][8] hTAAR1 and hTAAR2 are both required for white blood cell activation by trace amines in granulocytes.[9] Phenethylamine, para-tyramine, and 3-iodothyronamine are full agonists of hTAAR2 with EC50 values of 0.43 +/- 0.05 nM, 0.52 +/- 0.05 nM, and 0.25 +/- 0.04 nM respectively. A single nucleotide polymorphism nonsense mutation of the TAAR2 gene is associated with schizophrenia. TAAR2 is a probable pseudogene in 10-15% of Asians as a result of a polymorphism that produces a premature stop codon at amino acid 168.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
trace amine-associated receptor 2
NCBI Official Synonym Full Names
trace amine-associated receptor 2
NCBI Official Symbol
Taar2
NCBI Official Synonym Symbols
Gm224; Gm225; Gpr58; Tarr2
NCBI Protein Information
trace amine-associated receptor 2
UniProt Protein Name
Trace amine-associated receptor 2
UniProt Gene Name
Taar2
UniProt Synonym Gene Names
Gm225; Gpr58; TaR-2; Trace amine receptor 2; mTaar2
UniProt Entry Name
TAAR2_MOUSE
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Product Notes
The Mouse TAAR2 taar2 (Catalog #AAA332332) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332332 ELISA Kit recognizes Mouse TAAR2. It is sometimes possible for the material contained within the vial of "Trace Amine-Associated Receptor 2 (TAAR2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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