Standard Curve (Sample)
(Fig.1. Mouse Transforming growth factor beta2 (TGF-beta2) Standard Curve.)
Mouse Transforming Growth Factor beta2 (TGF-beta2) ELISA Kit | TGF-beta2 elisa kit
Mouse Transforming Growth Factor beta2 (TGF-beta2) ELISA Kit
Gene Names
Tgfb2; Tgfb-2; BB105277; Tgf-beta2
Reactivity
Mouse
Synonyms
Transforming Growth Factor beta2 (TGF-beta2); N/A; Mouse Transforming Growth Factor beta2 (TGF-beta2) ELISA Kit; TGFB2; MGC116892; TGF-beta2; TGF-beta2 elisa kit
Reactivity
Mouse
Specificity
Mouse Transforming growth factor beta2 (TGF-beta2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TGFB2. No significant cross-reactivity or interference between Mouse TGFB2 and analogues was observed.
Sequence Length
414
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Transforming growth factor beta2 (TGF-beta2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Transforming growth factor beta2 (TGF-beta2) Standard Curve.)
Related Product Information for TGF-beta2 elisa kit
Description: This Mouse Transforming growth factor beta2 (TGF-beta2) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFB2 in samples. An antibody specific for TGFB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGFB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tgfb2 encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Mice lacking a functional copy of this gene display developmental defects in multiple organs and perinatal lethality. Heterozygous mutant mice exhibit aortic root aneurysm. Tgfb2 encodes multiple isoforms that may undergo similar proteolytic processing.
Background: Tgfb2 encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Mice lacking a functional copy of this gene display developmental defects in multiple organs and perinatal lethality. Heterozygous mutant mice exhibit aortic root aneurysm. Tgfb2 encodes multiple isoforms that may undergo similar proteolytic processing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Transforming growth factor beta-2 proprotein
NCBI Official Synonym Full Names
transforming growth factor, beta 2
NCBI Official Symbol
Tgfb2
NCBI Official Synonym Symbols
Tgfb-2; BB105277; Tgf-beta2
NCBI Protein Information
transforming growth factor beta-2 proprotein; transforming growth factor beta-2
UniProt Protein Name
Transforming growth factor beta-2
UniProt Gene Name
Tgfb2
UniProt Synonym Gene Names
TGF-beta-2; LAP
UniProt Entry Name
TGFB2_MOUSE
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Product Notes
The Mouse TGF-beta2 tgfb2 (Catalog #AAA332378) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332378 ELISA Kit recognizes Mouse TGF-beta2. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor beta2 (TGF-beta2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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