Standard Curve (Sample)
(Fig.1. Mouse Transforming growth factor beta3 (TGF-beta3) Standard Curve.)
Mouse Transforming Growth Factor beta3 (TGF-beta3) ELISA Kit | TGF-beta3 elisa kit
Mouse Transforming Growth Factor beta3 (TGF-beta3) ELISA Kit
Gene Names
Tgfb3; Tgfb-3; TGF-beta-3
Reactivity
Mouse
Synonyms
Transforming Growth Factor beta3 (TGF-beta3); N/A; Mouse Transforming Growth Factor beta3 (TGF-beta3) ELISA Kit; TGFB3; ARVD; FLJ16571; TGF-beta3; TGF-beta3 elisa kit
Reactivity
Mouse
Specificity
Mouse Transforming growth factor beta3 (TGF-beta3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TGFB3. No significant cross-reactivity or interference between Mouse TGFB3 and analogues was observed.
Sequence Length
410
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Transforming growth factor beta3 (TGF-beta3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Transforming growth factor beta3 (TGF-beta3) Standard Curve.)
Related Product Information for TGF-beta3 elisa kit
Description: This Mouse Transforming growth factor beta3 (TGF-beta3) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFB3 in samples. An antibody specific for TGFB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGFB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TGF-beta3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others. TGF-beta3 is produced by chondrocytes, glial cells, astrocytes and some cancer cells. TGF-beta3 binds to TbetaRII homodimer, which then complexes with TbetaRI homodimer. The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes. Based on knock-out studies in mice, TGF-beta3 is critical for lung and palate development.
Background: TGF-beta3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others. TGF-beta3 is produced by chondrocytes, glial cells, astrocytes and some cancer cells. TGF-beta3 binds to TbetaRII homodimer, which then complexes with TbetaRI homodimer. The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes. Based on knock-out studies in mice, TGF-beta3 is critical for lung and palate development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Transforming growth factor beta-3 proprotein
NCBI Official Synonym Full Names
transforming growth factor, beta 3
NCBI Official Symbol
Tgfb3
NCBI Official Synonym Symbols
Tgfb-3; TGF-beta-3
NCBI Protein Information
transforming growth factor beta-3 proprotein
UniProt Protein Name
Transforming growth factor beta-3
UniProt Gene Name
Tgfb3
UniProt Synonym Gene Names
TGF-beta-3; LAP
UniProt Entry Name
TGFB3_MOUSE
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Product Notes
The Mouse TGF-beta3 tgfb3 (Catalog #AAA332379) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332379 ELISA Kit recognizes Mouse TGF-beta3. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor beta3 (TGF-beta3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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