Standard Curve (Sample)
(Fig.1. Mouse Translationally-controlled tumor protein (TPT1) Standard Curve.)
Mouse Translationally-Controlled Tumor Protein (TPT1) ELISA Kit | TPT1 elisa kit
Mouse Translationally-Controlled Tumor Protein (TPT1) ELISA Kit
Gene Names
Tpt1; Trt; p21; p23; TCTP
Reactivity
Mouse
Synonyms
Translationally-Controlled Tumor Protein (TPT1); N/A; Mouse Translationally-Controlled Tumor Protein (TPT1) ELISA Kit; TPT1; FLJ27337; HRF; TCTP; p02; fortilin; histamine-releasing factor; TPT1 elisa kit
Reactivity
Mouse
Specificity
Mouse Translationally-controlled tumor protein (TPT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TPT1. No significant cross-reactivity or interference between Mouse TPT1 and analogues was observed.
Sequence Length
172
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Translationally-controlled tumor protein (TPT1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Translationally-controlled tumor protein (TPT1) Standard Curve.)
Related Product Information for TPT1 elisa kit
Description: This Mouse Translationally-controlled tumor protein (TPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPT1 in samples. An antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TCTP, is an essential component of the tuberous sclerosis-RHEB pathway. Reducing Drosophila Tctp levels reduced cell size, cell number, and organ size, which mimics Drosophila Rheb mutant phenotypes. Drosophila Tctp is genetically epistatic to Tsc1 and Rheb, but acts upstream of S6K, a downstream target of Rheb. Drosophila Tctp directly associated with Rheb and displayed guanine nucleotide exchange activity with it in vivo and in vitro. Human TCTP showed similar biochemical properties compared to Drosophila Tctp and could rescue Drosophila Tctp mutant phenotypes, suggesting that the function of TCTP in the TSC pathway is evolutionarily conserved. Overexpression of TSAP6 increased the level of TPT1 in exosome preparations and consistently enhanced TPT1 secretion.
Background: TCTP, is an essential component of the tuberous sclerosis-RHEB pathway. Reducing Drosophila Tctp levels reduced cell size, cell number, and organ size, which mimics Drosophila Rheb mutant phenotypes. Drosophila Tctp is genetically epistatic to Tsc1 and Rheb, but acts upstream of S6K, a downstream target of Rheb. Drosophila Tctp directly associated with Rheb and displayed guanine nucleotide exchange activity with it in vivo and in vitro. Human TCTP showed similar biochemical properties compared to Drosophila Tctp and could rescue Drosophila Tctp mutant phenotypes, suggesting that the function of TCTP in the TSC pathway is evolutionarily conserved. Overexpression of TSAP6 increased the level of TPT1 in exosome preparations and consistently enhanced TPT1 secretion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
translationally-controlled tumor protein
NCBI Official Synonym Full Names
tumor protein, translationally-controlled 1
NCBI Official Symbol
Tpt1
NCBI Official Synonym Symbols
Trt; p21; p23; TCTP
NCBI Protein Information
translationally-controlled tumor protein
UniProt Protein Name
Translationally-controlled tumor protein
UniProt Gene Name
Tpt1
UniProt Synonym Gene Names
Trt; TCTP
UniProt Entry Name
TCTP_MOUSE
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Product Notes
The Mouse TPT1 tpt1 (Catalog #AAA332076) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332076 ELISA Kit recognizes Mouse TPT1. It is sometimes possible for the material contained within the vial of "Translationally-Controlled Tumor Protein (TPT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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