Mouse Transmembrane Inner Ear Expressed Protein (TMIE) ELISA Kit | TMIE elisa kit

Mouse Transmembrane Inner Ear Expressed Protein (TMIE) ELISA Kit

Cat. Num. AAA332171

• Gene Names: Tmie; sr; Mm.87012; 5131400L21Rik
• Reactivity: Mouse
• Synonyms: Transmembrane Inner Ear Expressed Protein (TMIE); N/A; Mouse Transmembrane Inner Ear Expressed Protein (TMIE) ELISA Kit; TMIE; DFNB6; transmembrane inner ear protein; TMIE elisa kit

• Reactivity: Mouse
• Specificity: Mouse Transmembrane inner ear expressed protein (TMIE) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMIE. No significant cross-reactivity or interference between Mouse TMIE and analogues was observed.
• Sequence Length: 153
• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids

• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Standard Curve (Sample)

(Fig.1. Mouse Transmembrane inner ear expressed protein (TMIE) Standard Curve.)

Related Product Information for TMIE elisa kit

Description: This Mouse Transmembrane inner ear expressed protein (TMIE) ELISA Kit employs a two-site sandwich ELISA to quantitate TMIE in samples. An antibody specific for TMIE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMIE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMIE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMIE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: TMIE encodes a transmembrane inner ear protein. This gene is one of multiple genes responsible for recessive non-syndromic deafness (DFNB), also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment. TMIE gene is expressed in many human tissues and encodes a transcript of approximately 2.5 kb. Repeated RACE experiments on cDNA from human retina and various other tissues yielded an approximately 1.7-kb transcript; no other TMIE transcripts were identified. Sequence analysis predicted an intracellular N terminus, 2 transmembrane regions separated by an extracellular loop, and an intracellular C terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 22122855
• NCBI GeneID: 20776
• NCBI Accession #: NP_666372.1
• NCBI GenBank Nucleotide #: NP_666372.1
• UniProt Accession #: Q8K467
• NCBI Official Full Name: transmembrane inner ear expressed protein
• NCBI Official Synonym Full Names: transmembrane inner ear
• NCBI Official Symbol: Tmie
• NCBI Official Synonym Symbols: sr; Mm.87012; 5131400L21Rik
• NCBI Protein Information: transmembrane inner ear expressed protein
• UniProt Protein Name: Transmembrane inner ear expressed protein
• UniProt Gene Name: Tmie
• UniProt Entry Name: TMIE_MOUSE