Standard Curve (Sample)
(Fig.1. Mouse Transmembrane protein 63B (TMEM63B) Standard Curve.)
Mouse Transmembrane Protein 63B (TMEM63B) ELISA Kit | TMEM63B elisa kit
Mouse Transmembrane Protein 63B (TMEM63B) ELISA Kit
Gene Names
Tmem63b; BC026370
Reactivity
Mouse
Synonyms
Transmembrane Protein 63B (TMEM63B); N/A; Mouse Transmembrane Protein 63B (TMEM63B) ELISA Kit; TMEM63B; C6orf110; DKFZp686N2275; RP3-421H19.2; OTTHUMP00000016502; TMEM63B elisa kit
Reactivity
Mouse
Specificity
Mouse Transmembrane protein 63B (TMEM63B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM63B. No significant cross-reactivity or interference between Mouse TMEM63B and analogues was observed.
Sequence Length
832
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Transmembrane protein 63B (TMEM63B) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Transmembrane protein 63B (TMEM63B) Standard Curve.)
Related Product Information for TMEM63B elisa kit
Description: This Mouse Transmembrane protein 63B (TMEM63B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM63B in samples. An antibody specific for TMEM63B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM63B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM63B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM63B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMEM63B belongs to the SPO75/TMEM63 family. 3 isoforms of the human protein are produced by alternative splicing. Membrane; Multi-pass membrane protein, The exact function of TMEM63A remains unknown. spo75 mutants displayed a very heterogeneous phenotype. The phenotypes of spores in spo75 cells ranged from those that had little or no spore wall material to ones that had a wild-type appearance. A transmembrane protein is a polytopic protein that spans the entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.
Background: TMEM63B belongs to the SPO75/TMEM63 family. 3 isoforms of the human protein are produced by alternative splicing. Membrane; Multi-pass membrane protein, The exact function of TMEM63A remains unknown. spo75 mutants displayed a very heterogeneous phenotype. The phenotypes of spores in spo75 cells ranged from those that had little or no spore wall material to ones that had a wild-type appearance. A transmembrane protein is a polytopic protein that spans the entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CSC1-like protein 2
NCBI Official Synonym Full Names
transmembrane protein 63b
NCBI Official Symbol
Tmem63b
NCBI Official Synonym Symbols
BC026370
NCBI Protein Information
CSC1-like protein 2
UniProt Protein Name
CSC1-like protein 2
UniProt Gene Name
Tmem63b
UniProt Entry Name
CSCL2_MOUSE
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Product Notes
The Mouse TMEM63B tmem63b (Catalog #AAA332180) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332180 ELISA Kit recognizes Mouse TMEM63B. It is sometimes possible for the material contained within the vial of "Transmembrane Protein 63B (TMEM63B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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