Standard Curve (Sample)
(Fig.1. Mouse Transmembrane protein 9B (TMEM9B) Standard Curve.)
Mouse Transmembrane Protein 9B (TMEM9B) ELISA Kit | TMEM9B elisa kit
Mouse Transmembrane Protein 9B (TMEM9B) ELISA Kit
Gene Names
Tmem9b; AW539847; D7H11orf15; 2310004K06Rik; ICRFP703B1614Q5.3
Reactivity
Mouse
Synonyms
Transmembrane Protein 9B (TMEM9B); N/A; Mouse Transmembrane Protein 9B (TMEM9B) ELISA Kit; TMEM9B; C11orf15; TMEM9B elisa kit
Reactivity
Mouse
Specificity
Mouse Transmembrane protein 9B (TMEM9B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM9B. No significant cross-reactivity or interference between Mouse TMEM9B and analogues was observed.
Sequence Length
199
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Transmembrane protein 9B (TMEM9B) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Transmembrane protein 9B (TMEM9B) Standard Curve.)
Related Product Information for TMEM9B elisa kit
Description: This Mouse Transmembrane protein 9B (TMEM9B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM9B in samples. An antibody specific for TMEM9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM9B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The intracellular signaling pathway by which TNF induces its pleiotropic actions is well-characterized and includes unique components, as well as modules shared with other signaling pathways. TMEM9B as an important component of TNF signaling and which is a module shared with the IL-1beta and Toll-like receptor (TLR) pathways. is a glycosylated protein localized in membranes of the lysosome and partially in early endosomes. The expression of TMEM9B is required for the production of proinflammatory cytokines induced by TNF, IL-1beta, and TLR ligands, but not for apoptotic cell death triggered by TNF or Fas ligand. TMEM9B is essential in TNF activation of both the NF-kappaB and MAPK pathways.TMEM9B is a key component of inflammatory signaling pathways and suggest that endosomal or lysosomal compartments regulate these pathways.
Background: The intracellular signaling pathway by which TNF induces its pleiotropic actions is well-characterized and includes unique components, as well as modules shared with other signaling pathways. TMEM9B as an important component of TNF signaling and which is a module shared with the IL-1beta and Toll-like receptor (TLR) pathways. is a glycosylated protein localized in membranes of the lysosome and partially in early endosomes. The expression of TMEM9B is required for the production of proinflammatory cytokines induced by TNF, IL-1beta, and TLR ligands, but not for apoptotic cell death triggered by TNF or Fas ligand. TMEM9B is essential in TNF activation of both the NF-kappaB and MAPK pathways.TMEM9B is a key component of inflammatory signaling pathways and suggest that endosomal or lysosomal compartments regulate these pathways.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transmembrane protein 9B
NCBI Official Synonym Full Names
TMEM9 domain family, member B
NCBI Official Symbol
Tmem9b
NCBI Official Synonym Symbols
AW539847; D7H11orf15; 2310004K06Rik; ICRFP703B1614Q5.3
NCBI Protein Information
transmembrane protein 9B
UniProt Protein Name
Transmembrane protein 9B
UniProt Gene Name
Tmem9b
UniProt Entry Name
TMM9B_MOUSE
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Product Notes
The Mouse TMEM9B tmem9b (Catalog #AAA332173) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332173 ELISA Kit recognizes Mouse TMEM9B. It is sometimes possible for the material contained within the vial of "Transmembrane Protein 9B (TMEM9B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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