Standard Curve (Sample)
(Fig.1. Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) Standard Curve.)
Mouse Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B) ELISA Kit | TNRC6B elisa kit
Mouse Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B) ELISA Kit
Gene Names
Tnrc6b; AI848765; 2700090M07Rik; A730065C02Rik; D230019K20Rik
Reactivity
Mouse
Synonyms
Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B); N/A; Mouse Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B) ELISA Kit; TNRC6B; RP5-1042K10.7; KIAA1093; OTTHUMP00000199175; TNRC6B elisa kit
Reactivity
Mouse
Specificity
Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNRC6B. No significant cross-reactivity or interference between Mouse TNRC6B and analogues was observed.
Sequence Length
1810
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) Standard Curve.)
Related Product Information for TNRC6B elisa kit
Description: This Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC6B in samples. An antibody specific for TNRC6B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNRC6B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC6B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC6B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 1,203-amino acid protein contains an RNA recognition motif. RT-PCR ELISA detected moderate expression in all tissues and specific brain regions examined, with highest expression in cerebellum. Schneider et al. (2006) stated that the 1,727-amino acid TNRC6B protein contains a glycine/tryptophan (GW)-rich N-terminal domain, a central glutamine-rich region, and a C-terminal RNA recognition motif. TNRC6B copurified with a cytoplasmic HeLa cell protein complex containing AGO2 (EIF2C2), DICER, and MOV10. Epitope-tagged TNRC6B coimmunoprecipitated with tagged AGO1 (EIF2C1) and tagged AGO2. TNRC6B was required to mediate microRNA-guided mRNA cleavage in HeLa cell culture.
Background: The deduced 1,203-amino acid protein contains an RNA recognition motif. RT-PCR ELISA detected moderate expression in all tissues and specific brain regions examined, with highest expression in cerebellum. Schneider et al. (2006) stated that the 1,727-amino acid TNRC6B protein contains a glycine/tryptophan (GW)-rich N-terminal domain, a central glutamine-rich region, and a C-terminal RNA recognition motif. TNRC6B copurified with a cytoplasmic HeLa cell protein complex containing AGO2 (EIF2C2), DICER, and MOV10. Epitope-tagged TNRC6B coimmunoprecipitated with tagged AGO1 (EIF2C1) and tagged AGO2. TNRC6B was required to mediate microRNA-guided mRNA cleavage in HeLa cell culture.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
trinucleotide repeat-containing gene 6B protein isoform 1
NCBI Official Synonym Full Names
trinucleotide repeat containing 6b
NCBI Official Symbol
Tnrc6b
NCBI Official Synonym Symbols
AI848765; 2700090M07Rik; A730065C02Rik; D230019K20Rik
NCBI Protein Information
trinucleotide repeat-containing gene 6B protein
UniProt Protein Name
Trinucleotide repeat-containing gene 6B protein
UniProt Gene Name
Tnrc6b
UniProt Synonym Gene Names
Kiaa1093
UniProt Entry Name
TNR6B_MOUSE
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Product Notes
The Mouse TNRC6B tnrc6b (Catalog #AAA332101) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332101 ELISA Kit recognizes Mouse TNRC6B. It is sometimes possible for the material contained within the vial of "Trinucleotide Repeat-Containing Gene 6B Protein (TNRC6B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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