Standard Curve (Sample)
(Fig.1. Mouse Troponin C, skeletal muscle (TNNC2) Standard Curve.)
Mouse Troponin C, Skeletal Muscle (TNNC2) ELISA Kit | TNNC2 elisa kit
Mouse Troponin C, Skeletal Muscle (TNNC2) ELISA Kit
Gene Names
Tnnc2; Tncs
Reactivity
Mouse
Synonyms
Troponin C, Skeletal Muscle (TNNC2); N/A; Mouse Troponin C, Skeletal Muscle (TNNC2) ELISA Kit; TNNC2; Fast skeletal muscle troponin C; troponin C2; fast; TNNC2 elisa kit
Reactivity
Mouse
Specificity
Mouse Troponin C, skeletal muscle (TNNC2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNNC2. No significant cross-reactivity or interference between Mouse TNNC2 and analogues was observed.
Sequence Length
160
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Troponin C, skeletal muscle (TNNC2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Troponin C, skeletal muscle (TNNC2) Standard Curve.)
Related Product Information for TNNC2 elisa kit
Description: This Mouse Troponin C, skeletal muscle (TNNC2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNC2 in samples. An antibody specific for TNNC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The troponin C protein is the calcium-binding subunit of the troponin complex. It occurs in 2 distinct isoforms: fast skeletal troponin C, which is expressed exclusively in fast-twitch skeletal muscle, and slow/cardiac troponin C, which is expressed in both cardiac and slow-twitch skeletal muscle. Gahlmann and Kedes (1990) described the structure and sequence of the gene for fast-twitch troponin C. By PCR-based analysis of a monochromosomal hybrid panel followed by analysis of the Genebridge 4 radiation hybrid panel, Townsend et al. (1997) mapped the TNNC2 gene to chromosome 20. Tiso et al. (1997) independently mapped the TNNC2 gene to 20q12-q13.11 using PCR of radiation hybrids.
Background: The troponin C protein is the calcium-binding subunit of the troponin complex. It occurs in 2 distinct isoforms: fast skeletal troponin C, which is expressed exclusively in fast-twitch skeletal muscle, and slow/cardiac troponin C, which is expressed in both cardiac and slow-twitch skeletal muscle. Gahlmann and Kedes (1990) described the structure and sequence of the gene for fast-twitch troponin C. By PCR-based analysis of a monochromosomal hybrid panel followed by analysis of the Genebridge 4 radiation hybrid panel, Townsend et al. (1997) mapped the TNNC2 gene to chromosome 20. Tiso et al. (1997) independently mapped the TNNC2 gene to 20q12-q13.11 using PCR of radiation hybrids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
troponin C, skeletal muscle
NCBI Official Synonym Full Names
troponin C2, fast
NCBI Official Symbol
Tnnc2
NCBI Official Synonym Symbols
Tncs
NCBI Protein Information
troponin C, skeletal muscle
UniProt Protein Name
Troponin C, skeletal muscle
UniProt Gene Name
Tnnc2
UniProt Synonym Gene Names
Tncs
UniProt Entry Name
TNNC2_MOUSE
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Product Notes
The Mouse TNNC2 tnnc2 (Catalog #AAA332115) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332115 ELISA Kit recognizes Mouse TNNC2. It is sometimes possible for the material contained within the vial of "Troponin C, Skeletal Muscle (TNNC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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