Mouse anti-Human Granzyme A Antibody | anti-GZMA antibody

Anti-Hu Granzyme A Purified

Cat. Num. AAA129182

• Gene Names: GZMA; HFSP; CTLA3
• Reactivity: Human
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay, Immunocytochemistry
• Purity: Purified by protein-A affinity chromatography.
• Synonyms: Granzyme A; N/A; Anti-Hu Granzyme A Purified; HFSP; CTLA3; GrA; anti-GZMA antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: CB9
• Specificity: The mouse monoclonal CB9 recognizes granzyme A, a 28kDa serine protease expressed intracellularly in activated Tc cells and NK cells.
• Purity/Purification: Purified by protein-A affinity chromatography.
• Form/Format: Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GZMA antibody: IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry)
• Immunogen: Full length human granzyme A
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Related Product Information for anti-GZMA antibody

Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA).

References

Choi BD, Gedeon PC, Herndon JE, Archer GE, Reap EA, Sanchez-Perez L, Mitchell DA, Bigner DD, Sampson JH: Human regulatory T cells kill tumor cells through granzyme-dependent cytotoxicity upon retargeting with a bispecific antibody. Cancer Immunol Res. 2013 Sep;1(3):163., URL: https://pubmed.ncbi.nlm.nih.gov/24570975/
Beresford PJ, Kam CM, Powers JC, Lieberman J: Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them. Proc Natl Acad Sci U S A 1997 Aug 19;94(17):9285-90, URL: https://pubmed.ncbi.nlm.nih.gov/9256474/
Mahrus S, Craik CS: Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 2005 May;12(5):567-77., URL: https://pubmed.ncbi.nlm.nih.gov/15911377/
Bratke K, Kuepper M, Bade B, Virchow JC, Luttmann W: Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur J Immunol. 2005 Sep;35(9):2608-16, URL: https://pubmed.ncbi.nlm.nih.gov/16106370/,

NCBI and Uniprot Product Information

• NCBI GI #: 5453676
• NCBI GeneID: 3001
• NCBI Accession #: NP_006135.1
• NCBI GenBank Nucleotide #: NM_006144.3
• UniProt Accession #: P12544; Q6IB36; A4PHN1
• Molecular Weight: 28,999 Da
• NCBI Official Full Name: granzyme A
• NCBI Official Synonym Full Names: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• NCBI Official Symbol: GZMA
• NCBI Official Synonym Symbols: HFSP; CTLA3
• NCBI Protein Information: granzyme A; HF; h factor; CTL tryptase; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease)
• UniProt Protein Name: Granzyme A
• UniProt Gene Name: GZMA
• UniProt Synonym Gene Names: CTLA3; HFSP; H factor; HF
• UniProt Entry Name: GRAA_HUMAN