ELISA
(Binding curve of anti-CD33 antibody hP76.6 (Gemtuzumab) (AAA72233-10.0) to human CD33 ELISA Plate coated with human His-tagged CD33 (Sino Biologicals, 12238-H08H) at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using AAA72233-10.0. For detection, a 1:4000 dilution of HRP-labelled anti-human kappa light chain antibody was used.)
Mouse anti-Human CD33 Antibody | anti-CD33 antibody
Anti-CD33 [hP67.6 (Gemtuzumab)], Mouse IgG1
Gene Names
CD33; p67; SIGLEC3; SIGLEC-3
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD33; N/A; Anti-CD33 [hP67.6 (Gemtuzumab)], Mouse IgG1; Anti-CD33 [hP67.6 (Gemtuzumab)]; Siglec-3; anti-CD33 antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1, Kappa
Clone Number
hP67.6 (Gemtuzumab)
Specificity
Recognises human CD33.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD33 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Gemtuzumab was generated by huminization of the murine P67.6 clone, raised against CD33-transfected FMY9S5 cells of human origin. CD33 is a transmembrane receptor expressed on predominantly on cells of the myeloid lineage, but it can also be found on some lymphoid cells.
Chimeric Use Statement
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
ELISA
(Binding curve of anti-CD33 antibody hP76.6 (Gemtuzumab) (AAA72233-10.0) to human CD33 ELISA Plate coated with human His-tagged CD33 (Sino Biologicals, 12238-H08H) at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using AAA72233-10.0. For detection, a 1:4000 dilution of HRP-labelled anti-human kappa light chain antibody was used.)
ELISA
(Binding curve of anti-CD33 antibody hP76.6 (Gemtuzumab) (AAA72233-10.0) to human CD33 ELISA Plate coated with human His-tagged CD33 (Sino Biologicals, 12238-H08H) at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using AAA72233-10.0. For detection, a 1:4000 dilution of HRP-labelled anti-human kappa light chain antibody was used.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-CD33 antibody hP67.6 (Gemtuzumab) (AAA72233) Human leukocytes were stained with an isotype control or the rabbit-chimeric version of hP67.6 (Gemtuzumab) (AAA72233-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-CD33 antibody hP67.6 (Gemtuzumab) (AAA72233) Human leukocytes were stained with an isotype control or the rabbit-chimeric version of hP67.6 (Gemtuzumab) (AAA72233-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)
Related Product Information for anti-CD33 antibody
A human IgG4 variant conjugated to derivative of the cytotoxic drug calicheamicin gamma 1, N-acetylcalicheamicin dimethyl hydrazide, has been used as a therapeutic against acute myeloid leukemia. This antibody may also be used in flow cytometry and immunofluorescence.
Product Categories/Family for anti-CD33 antibody
References
Appelbaum et al. The use of radiolabeled anti-CD33 antibody to augment marrow irradiation prior to marrow transplantation for acute myelogenous leukemia. Transplantation PMID:1440849
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 25kDa/33kDa/39kDa
Observed MW: 77kDa
Observed MW: 77kDa
NCBI Official Full Name
Myeloid cell surface antigen CD33
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
NCBI Protein Information
myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3
UniProt Protein Name
Myeloid cell surface antigen CD33
UniProt Gene Name
CD33
UniProt Synonym Gene Names
SIGLEC3; Siglec-3
UniProt Entry Name
CD33_HUMAN
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Product Notes
The CD33 cd33 (Catalog #AAA72233) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD33 [hP67.6 (Gemtuzumab)], Mouse IgG1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD33 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD33 cd33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD33, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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