Mouse anti-Human CD7 Antibody | anti-CD7 antibody

Anti-Hu CD7 PE-Cy7

Cat. Num. AAA129342

• Gene Names: CD7; GP40; TP41; Tp40; LEU-9
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD7; N/A; Anti-Hu CD7 PE-Cy7; CD7 molecule; GP40, TLA, TP41, LEU-9; anti-CD7 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1
• Clone Number: 124-1D1
• Specificity: The mouse monoclonal antibody 124-1D1 recognizes an extracellular epitope of CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.
• Purity/Purification: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Applicable Applications for anti-CD7 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: not available
• Conjugation: PE-Cy7
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD7 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) Alexa Fluor® 647 antibody (4ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD7 (124-1D1) Alexa Fluor® 647 antibody (4ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD7 antibody

CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia tests.

References

*Nishimura M, Takanashi M, Okazaki H, Satake M, Nakajima K: Role of CD7 expressed in lung microvascular endothelial cells as Fc receptor for immunoglobulin M. Endothelium. 2006 Jul-Aug;13(4):287-92., URL: https://pubmed.ncbi.nlm.nih.gov/16990185/,*Chang H, Yeung J, Brandwein J, Yi QL: CD7 expression predicts poor disease free survival and post-remission survival in patients with acute myeloid leukemia and normal karyotype. Leuk Res. 2007 Feb;31(2):157-62. Epub 2006 Jul 11., URL: https://pubmed.ncbi.nlm.nih.gov/16837044/,*Lam GK, Liao HX, Xue Y, Alam SM, Scearce RM, Kaufman RE, Sempowski GD, Haynes BF. Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma. J Clin Immunol. 2005 Jan;25(1):41-9., URL: https://pubmed.ncbi.nlm.nih.gov/15742156/,*Alaibac M, Pigozzi B, Belloni-Fortina A, Michelotto A, Saponeri A, Peserico A. CD7 expression in reactive and malignant human skin T-lymphocytes. Anticancer Res. 2003 May-Jun;23(3B):2707-10., URL: https://pubmed.ncbi.nlm.nih.gov/12894562/,
*Pelegrí C, Castell M, Serra M, Rabanal M, Rodríguez-Palmero M, Castellote C, Franch A: Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells. Clin Exp Immunol. 2001 Sep;125(3):470-7., URL: https://pubmed.ncbi.nlm.nih.gov/11531956/,*Vicario M, Amat C, Rivero M, Moretó M, Pelegrí C: Dietary glutamine affects mucosal functions in rats with mild DSS-induced colitis. J Nutr. 2007 Aug;137(8):1931-7. , URL: http://www.ncbi.nlm.nih.gov/pubmed/17634266 ,*Preston SG, Majtán J, Kouremenou C, Rysnik O, Burger LF, Cabezas Cruz A, Chiong Guzman M, Nunn MA, Paesen GC, Nuttall PA, Austyn JM: Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses. PLoS Pathog. 2013;9(6):e1003450., URL: https://pubmed.ncbi.nlm.nih.gov/23825947/,*van Dongen JJ, Lhermitte L, Bottcher S, Almeida J, van der Velden VH, Flores-Montero J, Rawstron A, Asnafi V, Lécrevisse Q, Lucio P, Mejstrikova E, Szczepa?ski T, Kalina T, de Tute R, Bruggemann M, Sedek L, Cullen M, Langerak AW, Mendonça A, Macintyre E, Martin-Ayuso M, Hrusak O, Vidriales MB, Orfao A; EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708): EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012 Sep;26(9):1908-75. doi: 10.1038/leu.2012.120., URL: http://www.ncbi.nlm.nih.gov/pubmed/22552007 ,

NCBI and Uniprot Product Information

• NCBI GI #: 5453613
• NCBI GeneID: 924
• NCBI Accession #: NP_006128.1
• NCBI GenBank Nucleotide #: NM_006137.6
• UniProt Accession #: P09564
• Molecular Weight: 25,409 Da
• NCBI Official Full Name: T-cell antigen CD7
• NCBI Official Synonym Full Names: CD7 molecule
• NCBI Official Symbol: CD7
• NCBI Official Synonym Symbols: GP40; TP41; Tp40; LEU-9
• NCBI Protein Information: T-cell antigen CD7; CD7 antigen (p41); T-cell leukemia antigen; T-cell surface antigen Leu-9; p41 protein
• UniProt Protein Name: T-cell antigen CD7
• UniProt Gene Name: CD7
• UniProt Entry Name: CD7_HUMAN