IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with anti-CEA (AAA72264) EB-022 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EB-022 (AAA72264-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72264-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse anti-Human CEA Antibody | anti-CEA antibody
Anti-CEA [EB-022], Mouse IgG1
Gene Names
CEACAM5; CEA; CD66e
Reactivity
Human
Applications
ELISA
Purity
Protein A affinity purified
Synonyms
CEA; N/A; Anti-CEA [EB-022], Mouse IgG1; Anti-CEA [EB-022]; Carcinoembryonic antigen; Carcinoembryonic antigen-related cell adhesion molecule 5; CEACAM5; Cluster of Differentiation 66e; CD66e; anti-CEA antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1, Kappa
Clone Number
EB-022
Specificity
EB-022 was shown by ELISA to react specifically with CEA (CD66e), as opposed to the related CD66 family member CD66c (also known as NCA; unpublished data).
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CEA antibody
ELISA
Immunogen
This antibody was generated by immunising BALB/c mice. Splenocytes from immunised mice were obtained and fused with myeloma cells to generate a hybridoma.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with anti-CEA (AAA72264) EB-022 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EB-022 (AAA72264-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72264-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF7 cells with anti-CEA (AAA72264) EB-022 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EB-022 (AAA72264-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72264-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-CEA antibody EB-022 (AAA72264). Human colorectal carcinoma (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of EB-022 (AAA72264-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CEA antibody EB-022 (AAA72264). Human colorectal carcinoma (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of EB-022 (AAA72264-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Related Product Information for anti-CEA antibody
EB-022 was used in ELISAs against CEA (CD66e) and NCA (CD66c) to show specificity of binding (unpublished data).
Product Categories/Family for anti-CEA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,795 Da
NCBI Official Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN
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Product Notes
The CEA ceacam5 (Catalog #AAA72264) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CEA [EB-022], Mouse IgG1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEA can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CEA ceacam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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