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Application Data
(AbFlex Histone H4K20me3 antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex Histone H4K20me3 antibody (rAb) antibody for Histone H4K20me3. Various methyl-lysine H4 or H3 peptides were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlex Histone H4K20me3antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)
Mouse Histone H4K20me3 Antibody | anti-H4K20me3 antibody
AbFlex Histone H4K20me3 antibody (rAb)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Ni-NTA
Synonyms
Histone H4K20me3; N/A; AbFlex Histone H4K20me3 antibody (rAb); anti-H4K20me3 antibody
Host
Mouse
Reactivity
Human, Wide Range Predicted
Isotype
IgG2a
Purity/Purification
Ni-NTA
Form/Format
Purified IgG in 200mM Hepes pH7.5, 100mM NaCl, 50mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
0.82ug/ul (varies by lot)
Applicable Applications for anti-H4K20me3 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Antibody Type
Recombinant Antibody
Conjugation
Methylated
Immunogen
This Histone H4 trimethyl Lys20 antibody (rAb) was raised against a peptide containing trimethyl Lys20 of Histone H4.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Application Data
(AbFlex Histone H4K20me3 antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex Histone H4K20me3 antibody (rAb) antibody for Histone H4K20me3. Various methyl-lysine H4 or H3 peptides were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlex Histone H4K20me3antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)
Application Data
(AbFlex Histone H4K20me3 antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex Histone H4K20me3 antibody (rAb) antibody for Histone H4K20me3. Various methyl-lysine H4 or H3 peptides were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlex Histone H4K20me3antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)
WB (Western Blot)
(AbFlex Histone H4K20me3 antibody (rAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with AbFlex Histone H4K20me3 antibody (rAb) ) (2 ug/ml dilution).)
WB (Western Blot)
(AbFlex Histone H4K20me3 antibody (rAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with AbFlex Histone H4K20me3 antibody (rAb) ) (2 ug/ml dilution).)
IF (Immunofluorescence)
(AbFlex H4K20me3 antibody (rAb) tested by immunofluorescence. HeLa cell stained with 2ug /mL of AbFlex H4K20me3 antibody (rAb) followed by anti-mouse-IgG-488.)
IF (Immunofluorescence)
(AbFlex H4K20me3 antibody (rAb) tested by immunofluorescence. HeLa cell stained with 2ug /mL of AbFlex H4K20me3 antibody (rAb) followed by anti-mouse-IgG-488.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex Histone H4K20me3 Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from T-47D, mammary epithelial ductal cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H4K20me3 binding sites. The image shows binding across a region of chromosome 19.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex Histone H4K20me3 Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from T-47D, mammary epithelial ductal cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H4K20me3 binding sites. The image shows binding across a region of chromosome 19.)
Related Product Information for anti-H4K20me3 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Histone H4K20me3 antibody (rAb) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, ELISA, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K20me3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K20me3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.
Product Categories/Family for anti-H4K20me3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,367 Da
NCBI Official Full Name
histone H4
NCBI Official Synonym Full Names
histone cluster 4, H4
NCBI Official Symbol
HIST4H4
NCBI Official Synonym Symbols
H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
NCBI Protein Information
histone H4; histone 4, H4; OTTHUMP00000239323
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
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Product Notes
The H4K20me3 hist1h4a (Catalog #AAA60256) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Histone H4K20me3 antibody (rAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4K20me3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the H4K20me3 hist1h4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4K20me3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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