FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from IgG (Fab) negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgG (Fab) Biotin antibody (concentration in sample 0.56ug/ml, Streptavidin APC).)
Mouse anti-Human IgG (Fab) Antibody | anti-IgG antibody
Anti-Hu IgG (Fab) Biotin
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Synonyms
IgG (Fab); N/A; Anti-Hu IgG (Fab) Biotin; Immunoglobulin G Fab fragment; anti-IgG antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG2b
Clone Number
4A11
Specificity
The mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG. This antibody detects Fab fragments regardless light chain type.
Purity/Purification
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Form/Format
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IgG antibody
ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry)
Immunogen
Purified human IgG.
Conjugation
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from IgG (Fab) negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgG (Fab) Biotin antibody (concentration in sample 0.56ug/ml, Streptavidin APC).)
FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from IgG (Fab) negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgG (Fab) Biotin antibody (concentration in sample 0.56ug/ml, Streptavidin APC).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgG (Fab) Biotin antibody (concentration in sample 0.56ug/ml, Streptavidin APC).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgG (Fab) Biotin antibody (concentration in sample 0.56ug/ml, Streptavidin APC).)
Related Product Information for anti-IgG antibody
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
References
*Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/8258141 ordina,*Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/6966908 ordina,*Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/1122610 ordina,*Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690 ordinalp,
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Product Notes
The IgG (Catalog #AAA129335) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu IgG (Fab) Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgG (Fab) can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (Fab), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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