IF (Immunofluorescence)
(Figure 3. Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin. Blue. DRAQ5 fluorescent DNA dye)
Mouse anti-Human, Mouse BRAF Antibody | anti-BRAF antibody
BRAF Antibody
Gene Names
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
BRAF; N/A; BRAF Antibody; BRAF1; RAFB1; B-RAF1; FLJ95109; anti-BRAF antibody
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG1
Clone Number
1H12
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
766
Applicable Applications for anti-BRAF antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of human BRAF expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
IF (Immunofluorescence)
(Figure 3. Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin. Blue. DRAQ5 fluorescent DNA dye)
IF (Immunofluorescence)
(Figure 3. Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin. Blue. DRAQ5 fluorescent DNA dye)
IHC (Immunohiostchemistry)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF mouse mAb.)
IHC (Immunohiostchemistry)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF mouse mAb.)
WB (Western Blot)
(Figure 1. Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate. Figure 1.)
WB (Western Blot)
(Figure 1. Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate. Figure 1.)
Related Product Information for anti-BRAF antibody
Cytokeratin 5, also known as CK5, KRT5. It is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.
Product Categories/Family for anti-BRAF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
87kDa
NCBI Official Full Name
serine/threonine-protein kinase B-raf isoform 1
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
BRAF
NCBI Official Synonym Symbols
NS7; B-raf; BRAF1; RAFB1; B-RAF1
NCBI Protein Information
serine/threonine-protein kinase B-raf
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Gene Name
BRAF
UniProt Synonym Gene Names
BRAF1; RAFB1
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Product Notes
The BRAF braf (Catalog #AAA108737) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BRAF Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRAF can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BRAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRAF, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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