WB (Western Blot)
(AbFlex HDAC1 antibody (rAb) tested by Western Blot. 30 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex HDAC1 antibody at 1 ug/ml.)
Mouse anti-Human, Mouse HDAC1 Antibody | anti-HDAC1 antibody
AbFlex HDAC1 antibody (rAb)
Gene Names
HDAC1; HD1; RPD3; GON-10; RPD3L1
Reactivity
Human, Mouse
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
HDAC1; N/A; AbFlex HDAC1 antibody (rAb); Histone Deacetylase 1; HD1; RPD3; GON-10; RPD3L1; anti-HDAC1 antibody
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-HDAC1 antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation)
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a KLH-conjugated peptide corresponding to amino acids 467-482 of human and murine HDAC1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(AbFlex HDAC1 antibody (rAb) tested by Western Blot. 30 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex HDAC1 antibody at 1 ug/ml.)
WB (Western Blot)
(AbFlex HDAC1 antibody (rAb) tested by Western Blot. 30 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex HDAC1 antibody at 1 ug/ml.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex HDAC1 antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse testes and 4 ug of AbFlex HDAC1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.3 million normalized sequence tags were mapped to identify HDAC1 binding sites on chromosome 1.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex HDAC1 antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from mouse testes and 4 ug of AbFlex HDAC1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.3 million normalized sequence tags were mapped to identify HDAC1 binding sites on chromosome 1.)
Related Product Information for anti-HDAC1 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex HDAC1 antibody (rAb) was raised in a Mouse host. It has been validated for use in ChIP-Seq and Western blot; it has been shown to react with Human and Mouse samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex HDAC1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex HDAC1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.
Product Categories/Family for anti-HDAC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 53 kDa
Observed: 54 kDa
Observed: 54 kDa
NCBI Official Full Name
histone deacetylase 1
NCBI Official Synonym Full Names
histone deacetylase 1
NCBI Official Symbol
HDAC1
NCBI Official Synonym Symbols
HD1; RPD3; GON-10; RPD3L1
NCBI Protein Information
histone deacetylase 1; reduced potassium dependency, yeast homolog-like 1
UniProt Protein Name
Histone deacetylase 1
UniProt Gene Name
HDAC1
UniProt Synonym Gene Names
RPD3L1; HD1
UniProt Entry Name
HDAC1_HUMAN
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Product Notes
The HDAC1 hdac1 (Catalog #AAA60275) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex HDAC1 antibody (rAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the HDAC1 hdac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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