Mouse CD107a Antibody | anti-CD107a antibody

Anti-Hu CD107a PE-Cy7

Cat. Num. AAA129379

• Gene Names: LAMP1; LAMPA; CD107a; LGP120
• Reactivity: Human, Non-human primates, Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD107a; N/A; Anti-Hu CD107a PE-Cy7; lysosomal associated membrane protein 1; LAMP-1, LAMPA; anti-CD107a antibody

• Host: Mouse
• Reactivity: Human, Non-human primates, Mouse
• Isotype: Mouse IgG1 kappa
• Clone Number: H4A3
• Specificity: The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
• Purity/Purification: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Applicable Applications for anti-CD107a antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Human PBMC
• Conjugation: PE-Cy7
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD107a positive CD203c positive basophils (red-filled) from CD107a negative CD203c negative mononuclear cells (black-dashed) in flow cytometry analysis (surface staining) of anti-IgE antibody stimulated human peripheral whole blood stained using anti-human CD107a (H4A3) PE-Cy7 antibody (10ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining pattern of anti-IgE antibody stimulated human peripheral blood mononuclear cells stained using anti-human CD107a (H4A3) PE-Cy7 antibody (10ul reagent/100ul of peripheral whole blood) and anti-human CD203c (NP4D6) APC antibody (10ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of anti-IgE antibody stimulated human peripheral whole blood stained using anti-human CD107a (H4A3) PE-Cy7 antibody (10ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD107a antibody

CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.

References

*Grutzkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15351990?ordin,*Barrachina M, Maes T, Buesa C, Ferrer I: Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 2006 Oct;32(5):505-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/16972884?ordin,*Sarafian VS, Marinova TT: Lysosomal membrane-associated glycoproteins are differentially expressed in acute and chronic human thymic involution. Acta Biol Hung. 2006 Sep;57(3):315-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/17048695?ordin,*Eskelinen EL: Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Mol Aspects Med. 2006 Oct-Dec;27(5-6):495-502., URL: https://www.ncbi.nlm.nih.gov/pubmed/16973206?ordin,*Hunyh KK, Eskelinen EL, Scott CC, Malevanets A, Saftig P, Grinstein S: LAMP proteins are required for fusion of lysosomes with phagosomes. EMBO J. 2007 Jan 24;26(2):313-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/17245426?ordin,
*Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95., URL: https://pubmed.ncbi.nlm.nih.gov/22365987/,*Mao H, Tu W, Liu Y, Qin G, Zheng J, Chan PL, Lam KT, Peiris JS, Lau YL: Inhibition of human natural killer cell activity by influenza virions and hemagglutinin. J Virol. 2010 May;84(9):4148-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/20164232 ,*Yu CI, Gallegos M, Marches F, Zurawski G, Ramilo O, García-Sastre A, Banchereau J, Palucka AK: Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines. Blood. 2008 Nov 1;112(9):3671-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18713944,*Tomescu C, Chehimi J, Maino VC, Montaner LJ: NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells. J Immunol. 2007 Aug 15;179(4):2097-104., URL: https://www.ncbi.nlm.nih.gov/pubmed/17675468,*Carlsten M, Bjorkstrom NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/17283169,*Marcenaro S, Gallo F, Martini S, Santoro A, Griffiths GM, Aricó M, Moretta L, Pende D: Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease. Blood. 2006 Oct 1;108(7):2316-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/16778144,*Deetz CO, Hebbeler AM, Propp NA, Cairo C, Tikhonov I, Pauza CD: Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys. Infect Immun. 2006 Aug;74(8):4505-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/16861636,*Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, Himeno M, Hamilton SR, August JT: Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases. Am J Pathol. 2001 Aug;159(2):449-55., URL: https://www.ncbi.nlm.nih.gov/pubmed/11485903,*Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, August JT: Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989 Jan;268(1):360-78., URL: https://pubmed.ncbi.nlm.nih.gov/2912382/,

NCBI and Uniprot Product Information

• NCBI GI #: 112380628
• NCBI GeneID: 3916
• NCBI Accession #: NP_005552.3
• NCBI GenBank Nucleotide #: NM_005561.3
• UniProt Accession #: P11279; Q8WU33; Q96I40; Q9BRD2; Q9NP13
• Molecular Weight: 44,882 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 1
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 1
• NCBI Official Symbol: LAMP1
• NCBI Official Synonym Symbols: LAMPA; CD107a; LGP120
• NCBI Protein Information: lysosome-associated membrane glycoprotein 1; LAMP-1; CD107 antigen-like family member A; lysosome-associated membrane protein 1
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 1
• UniProt Gene Name: LAMP1
• UniProt Synonym Gene Names: LAMP-1
• UniProt Entry Name: LAMP1_HUMAN