FCM/FACS (Flow Cytometry)
(Separation of human CD207 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4)(red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10ul reagent per milion cells in 100ul of cell suspension).)
Mouse CD207 Antibody | anti-CD207 antibody
Anti-Hu CD207 PE
Gene Names
CD207; CLEC4K
Reactivity
Human, Non-human primates
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Synonyms
CD207; N/A; Anti-Hu CD207 PE; CD207 molecule; CLEC4K, langerin; anti-CD207 antibody
Host
Mouse
Reactivity
Human, Non-human primates
Isotype
Mouse IgG1 lambda
Clone Number
2G3
Specificity
The mouse monoclonal antibody 2G3 recognizes an extracellular/luminal epitope of CD207, a transmembrane protein selectively expressed by Langerhans cells and by some other dendritic cell subsets. This antibody clone can target vaccine-peptides to dendritic cells.
Purity/Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Applicable Applications for anti-CD207 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Fusion protein of human CD207 extracellular part and IgG Fc fragment
Conjugation
PE
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human CD207 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4)(red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10ul reagent per milion cells in 100ul of cell suspension).)
FCM/FACS (Flow Cytometry)
(Separation of human CD207 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4)(red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10ul reagent per milion cells in 100ul of cell suspension).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10ul reagent per milion cells in 100ul of cell suspension) and anti-human CD11c (BU15) APC antibody (10ul reagent per milion cells in 100ul of cell suspension).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10ul reagent per milion cells in 100ul of cell suspension) and anti-human CD11c (BU15) APC antibody (10ul reagent per milion cells in 100ul of cell suspension).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10ul reagent per milion cells in 100ul of cell suspension).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) PE antibody (10ul reagent per milion cells in 100ul of cell suspension).)
Related Product Information for anti-CD207 antibody
CD207 is a 40 kDa type II transmembrane glycoprotein of the C-type lectin family. It binds to mannose-bearing glycoproteins and glycolipids of microbial and viral antigens, including HIV gp120. CD207 is expressed only in Langerhans cells, which are immature dendritic cells of the epidermis and mucosa, and its expression decreases during Langerhans cells maturation. Upon antigen binding CD207 localizes to the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed membranes, which leads to a nonclassical antigen-processing pathway.
References
*Epaulard O, Adam L, Poux C, Zurawski G, Salabert N, Rosenbaum P, Dereuddre-Bosquet N, Zurawski S, Flamar AL, Oh S, Romain G, Chapon C, Banchereau J, Lévy Y, Le Grand R, Martinon F: Macrophage- and neutrophil-derived TNF-alpha instructs skin langerhans cells to prime antiviral immune responses. J Immunol. 2014 Sep 1;193(5):2416-26. , URL: https://pubmed.ncbi.nlm.nih.gov/25057007/,*Adam L, Tchitchek N, Todorova B, Rosenbaum P, Joly C, Poux C, Chapon C, Spetz AL, Ustav M, Le Grand R, Martinon F: Innate molecular and cellular signature in the skin preceding long-lasting T cell responses after electroporated DNA vaccination. J Immunol. 2020 Jun 15;204(12):3375-3388., URL: https://pubmed.ncbi.nlm.nih.gov/32385135/,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,725 Da
NCBI Official Full Name
C-type lectin domain family 4 member K
NCBI Official Synonym Full Names
CD207 molecule
NCBI Official Symbol
CD207
NCBI Official Synonym Symbols
CLEC4K
NCBI Protein Information
C-type lectin domain family 4 member K
UniProt Protein Name
C-type lectin domain family 4 member K
UniProt Gene Name
CD207
UniProt Synonym Gene Names
CLEC4K
UniProt Entry Name
CLC4K_HUMAN
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Product Notes
The CD207 cd207 (Catalog #AAA129368) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD207 PE reacts with Human, Non-human primates and may cross-react with other species as described in the data sheet. AAA Biotech's CD207 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD207 cd207 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD207, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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