IF (Immunofluorescence)
(Immunofluorescence staining of Kelly cells with anti-Tau Oligomeric complex (AAA72289) TOC1 Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric rabbit IgG version of TOC1 (AAA72289-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72289-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse anti-Human Tau Oligomeric complex Antibody | anti-IgG2b antibody
Anti-Tau Oligomeric complex [TOC1], Mouse IgG2b
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Protein A affinity purified
Synonyms
Tau Oligomeric complex; N/A; Anti-Tau Oligomeric complex [TOC1], Mouse IgG2b; Anti-Tau Oligomeric complex [TOC1]; TOC-1; anti-IgG2b antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG2b, Kappa
Clone Number
TOC1
Specificity
This antibody is specific for amino acids 209-224 of the Tau Oligomeric complex.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IgG2b antibody
ELISA, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
The original antibody was generated by immunizing a mouse with electro eluted cross-linked Tau dimers.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of Kelly cells with anti-Tau Oligomeric complex (AAA72289) TOC1 Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric rabbit IgG version of TOC1 (AAA72289-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72289-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Kelly cells with anti-Tau Oligomeric complex (AAA72289) TOC1 Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric rabbit IgG version of TOC1 (AAA72289-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72289-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-Tau Oligomeric complex antibody TOC1 (AAA72289). Kelly(A) (0.5ug/ml) and U251(B) (0.5ug/ml) cells lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of TOC1 (AAA72289-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-Tau Oligomeric complex antibody TOC1 (AAA72289). Kelly(A) (0.5ug/ml) and U251(B) (0.5ug/ml) cells lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of TOC1 (AAA72289-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Related Product Information for anti-IgG2b antibody
Western blot was performed on recombinant Tau and human brain homogenate using this antibody (US8697076). This antibody was used for immunogold labeling on aggregated tau samples (US8697076) (Ward et al, 2013; pmid:23979027). Tissue from the entorhinal cortex and the hippocampus of Alzheimer's patients was used for immunohistochemistry using this antibody (US8697076). Tissue from the entorhinal cortex and the hippocampus of Alzheimer's patients was used for immunofluorescence with this antibody (US8697076). Western blot was performed on different deletion mutants of the Tau protein using this antibody (Ward et al, 2013; pmid:23979027). Immunohistochemistry was performed on tissue sections of severe AD, CBD, and PSP cases from the entorhinal cortex using this antibody (Ward et al, 2013; pmid:23979027). M1C cells were used for immunocytochemistry using this antibody (Shirafuji et al, 2018; pmid:29562600). Proteins from M1C cells were blotted with this antibody (Shirafuji et al, 2018; pmid:29562600). ELISA was performed on S1 fractions from a 9 month old murine PS19 cortex using this antibody (Apicco et al, 2017; pmid:29273772).
Product Categories/Family for anti-IgG2b antibody
References
Ward et al. TOC1: characterization of a selective oligomeric tau antibody. J Alzheimers Dis. 2013;37(3):593-602. PMID:2.40E+07
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Product Notes
The IgG2b (Catalog #AAA72289) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Tau Oligomeric complex [TOC1], Mouse IgG2b reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tau Oligomeric complex can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the IgG2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tau Oligomeric complex, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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